不同生长速率花鲈肌肉转录组分析及生长相关基因筛选

Muscle Transcriptome Analysis and Growth-Related Genes Screening of Lateolabrax maculatus with Different Growth Rate

【目的】筛选与花鲈肌肉生长相关的基因和信号通路,为揭示花鲈肌肉生长发育的遗传机制提供理论依据。【方法】分别取4尾生长快速[体长(34.22±2.53)cm]和4尾生长慢速[体长(17.46±1.78)cm]的花鲈个体肌肉进行转录组测序。用DESeq2 R软件包筛选差异表达基因(Differentially expressed genes,DEGs),对DEGs进行GO富集和KEGG富集分析。通过实时荧光定量PCR验证转录组数据的准确性。【结果与结论】测序原始数据经过质量控制和组装共得到137960个unigenes,其中68334个unigenes在Nr中注释成功。获得10552个DEGs,其中2064个DEGs表达量上调,8488个DEGs表达量下调,鉴定出胰岛素样生长因子结合蛋白1基因(IGFBP1)、纤维细胞生长因子基因(FGF)、肌肉生长抑制素基因(MSTN)、生长激素受体1基因(GHR1)等与肌肉生长发育相关的基因。DEGs的GO富集结果显示,与物质和能量代谢相关的条目差异显著。KEGG富集结果显示,糖酵解/合成、腺苷酸活化蛋白激酶(AMPK)、脂肪酸降解、精氨酸和脯氨酸代谢等信号通路与肌肉生长密切相关。

【Objective】This study aimed to screen candidate genes and signaling pathways that affect the muscle growth of Lateolabrax maculatus,providing a theoretical basis for elucidating the genetic mechanism of the growth and development of muscle of the fish.【Method】The skeletal muscle tissue were clipped from four fast-growing individuals(34.22±2.53 cm,body length)and four slow-growing individuals(17.46±1.78 cm,body length)for transcriptome sequencing.Differentially expressed genes(DEGs)were identified with DESeq2 R package firstly,and then were analyzed by Gene Ontology(GO)and KEGG enrichment.The transcriptome data were confirmed by real-time quantitative PCR.【Result and Conclusion】The raw data of sequencing obtained a total of 137960 unigenes of wich68334 unigenes were annotated in Nr database successfully.A total of 10552 DEGs were identified,of which 2064 DEGs were up-regulated and 8488 DEGs were down regulated.A few genes are related to growth of muscle,such as insulin-like growth factor-binding protein 1(IGFBP1),fibroblast growth factor(FGF),myostatin(MSTN),growth hormone receptor 1(GHR1),etc.The result of GO richment of DEGs showed that the term about material and energy metabolism was significance of difference.The result of KEGG showed that some signal pathways,including glycolysis/gluconeogenesis,arginine and proline metabolism,adenosine monophosphate activated protein kinase(AMPK)and fatty acid degradation,etc,were related closely with muscle growth and development.