草莓茎尖组织培养技术研究

Study on the Tip-shoot Tissue Culture Techniques for Strawberry

为建立完善的草莓组培快繁体系,解决种苗退化问题,以当地草莓主栽品种红颜茎尖为试材,设置0.1%升汞和0.1%升汞+2%吐温-80溶液2种外植体消毒处理,以MS为基本培养基,分别添加不同浓度6-苄氨基腺嘌呤(6-BA)和萘乙酸(NAA)的6个诱导和继代增殖培养基、5个生根培养基,研究草莓茎尖外植体消毒最佳方式与时间,筛选诱导、继代增殖及生根培养的最佳培养基。结果表明,0.1%升汞+2%吐温-80溶液处理8 min时草莓茎尖污染率及褐化率较低,萌发率最高,可达82.3%,为最佳的消毒方式。外植体在MS+1.0 mg/L 6-BA+0.2 mg/L NAA培养基中,可诱导出更多的健壮不定芽,萌发率为83.3%,为最佳诱导培养基;适宜的继代增殖培养基为MS+1.0mg/L 6-BA+0.02 mg/LNAA,增殖系数为4.5%;1/2MS+0.01 mg/L NAA为最佳生根培养基,生根率高达100%。

In order to improve the strawberry tissue culture and rapid propagation system and to address the quality degradation issue in seedlings,taking tip-shoots of a main strawberry variety Hongyan as material,two explant disinfection methods were used i.e.,0.1%HgClsolution and 0.1%HgClplus 2%Tween-80 solution,6 induction media and multiplicative culture media and 5 rooting culture media based on MS culture base plus different concentrations of 6-BA and NAA were formulated to study the optimum explant disinfection method and time,and to select the optimum culture media for induction,multiplicative culture and rooting culture for tip-shoot tissue culture of strawberry,respectively.Results showed that 0.1%HgClplus 2%Tween-80 solution treated for 8 min had the lowest pollution rate and browning rate of tip-shoot tissue,and the highest germination rate of 82.3%,which was considered as the optimum explant disinfection method.The optimum induction medium was MS plus 1.0 mg/L 6-BA plus 0.2 mg/L NAA which could induce more health adventitious buds with a germination rate of 83.3%.The optimum medium for multiplicative culture was MS plus 1 mg/L 6-BA plus 0.02 mg/L NAA which had a multiplication coefficient of 4.5%.The optimal medium for rooting was 1/2MS plus 0.01 mg/L NAA which showed a rooting rate of 100%.