黄大茶水提物改善高脂小鼠脂肪组织的脂肪酸代谢

Large-Leaf Yellow Tea Aqueous Extract Ameliorates Fatty Acid Metabolism in the Adipose Tissue of Obese Mice

目的:探究黄大茶水提物对高脂饮食小鼠脂肪组织脂肪酸代谢的调控机制。方法:将5周龄雄性C57BL/6小鼠随机分为对照组、高脂饮食组、高脂饮食+2.5%(终质量分数,下同)黄大茶水提取物组、高脂饮食+0.5%黄大茶水提取物组。饮食干预处理12周后,测定小鼠体质量、脂肪组织质量,观察脂肪组织形态,并分析脂肪酸代谢关键基因及蛋白表达量等指标。结果:2.5%黄大茶水提物能高度显著降低高脂小鼠体质量及脂肪组织质量(P<0.001);减少脂肪组织的脂质沉积;促进SREBP-1C、FAS、ACC、SCD-1等脂肪酸生成相关基因的表达,促进脂肪酸分解相关基因PGC-1α和CPT-1的表达;不同程度激活腺苷酸活化蛋白激酶(adenosine monophosphate activated protein kinase,AMPK)/乙酰辅酶A羧化酶(acetyl CoA-carboxylase,ACC)通路。结论:2.5%黄大茶水提物饮食干预能显著缓解高脂饮食小鼠肥胖和减少脂质沉积,促进脂肪组织脂肪酸合成和氧化分解代谢,此作用与黄大茶水提物激活AMPK/ACC通路相关。

Objective:To explore the regulatory mechanism of large-leaf yellow tea aqueous extract on fatty acid metabolism in the adipose tissue of high-fat diet fed mice.Methods:Five-week-old male C57BL/6 mice were randomly divided into four groups:control(fed on a low-fat diet(LFD)),high-fat diet(HFD),high-fat diet+2.5%large-leaf yellow tea aqueous extract(HFD+2.5%YT),and high-fat diet+0.5%large-leaf yellow tea aqueous extract(HFD+0.5%YT).The body mass,adipose tissue mass and morphology,and the expression of key genes and proteins associated with fatty acid metabolism in each group of mice were measured after dietary intervention for 12 weeks.Results:Large-leaf yellow tea aqueous extract at a concentration of 2.5%significantly reduced the body mass and adipose tissue mass of HFD fed mice(P<0.001),reduced lipid accumulation and promoted the expression of fatty acid synthesis genes such as SREBP-1C,FAS,ACC,SCD-1,and the the expression of fatty acid degradation genes such as PGC-1αand CPT-1.Moreover,large-leaf yellow tea aqueous extract activated the adenosine monophosphate activated protein kinase/acetyl CoA-carboxylase(AMPK/ACC)signaling pathway.Conclusion:Intervention with 2.5%large-leaf yellow tea aqueous extract prevents obesity and lipid deposition induced by high-fat diet,and promotes fatty acid synthesis and oxidative catabolism in adipose tissue through activating the AMPK/ACC signaling pathway.