正交试验优选紫白栓剂中紫草的提取工艺

Orthogonal Test to Optimize the Extraction Process of Arnebia euchroma in ZiBai Suppository

目的优选紫白栓中紫草的提取工艺。方法以羟基萘醌总色素含量为指标,采用正交试验L_(9)(3^(4))考察药材粒度、乙醇浓度、乙醇用量,筛选得出紫草的最佳提取工艺,并采用紫外-可见分光光度法测定含量。结果紫草的最佳提取工艺为过20目筛,95%乙醇8倍药量,超声40 min。结论该提取工艺操作简便、稳定可行。

OBJECTIVE To optimize the extraction process of Arnebia euchroma in ZiBai suppository.METHODS With hydroxy naphthoquinone total pigment as an indicator,the orthogonal test was used to investigate the particle size,ethanol concentration,ethanol consumption,and to select the best extraction process for Arnebia euchroma.And the content was determined by ultraviolet-visible spectrophotometry.RESULTS The best extraction technology of Arnebia euchroma was as follows:pass through 20 mesh sieve,95%ethanol,8 times the dosage,ultrasonic 40 min.CONCLUSION This extraction process is simple,stable and feasible.