米槁根际细菌对果实药用活性成分的影响及其PICRUST功能预测分析

Effects of rhizobacteria of Cinnamomum migao H. W. Li on medicinal active ingredients in fruit and its PICRUST function predictive analysis

研究道地药材米槁根际土壤细菌与果实活性成分含量间的关系,对米槁培育管理和果实活性成分增产有重要作用。采集黔、滇、桂3省交界处的米槁根际土壤与果实,利用高通量测序技术对米槁根际土壤细菌进行测序,同时测定土壤的理化特性和米槁果实的活性成分。结果表明,产自天峨的1,8-桉叶素、α-松油醇、柠檬烯含量最多,望谟的香桧烯含量最高;Shannon指数在望谟最高,为6.3158。几个采样点的优势菌门一致,为变形菌门、酸杆菌门、放线菌门和绿弯菌门,但优势菌属不同;在属水平上,优势菌属主要受pH值、氮含量的影响,大多数菌属与活性成分呈正相关;PICRUST功能预测结果表明,活性成分主要受细菌能量与物质的转运与代谢功能的影响,说明土壤中pH值和氮可通过影响根际土壤细菌的多样性,从而影响果实中药用活性成分的积累。

Research on the relationship between soil rhizobacteria of authentic medicinal material Cinnamomum migao and the fruit medicinal active ingredient plays an important role on C.migao cultivation and management and yield improvement of active components.Rhizosphere soils and fruits of C.migao at the junction of Guizhou,Yunnan and Guangxi were collected.High-throughput sequencing technology was used to analyze the gene sequences of soil rhizobacteria of C.migao,soil physicochemical properties and fruit active components were determined as well.As a result,fruit active components 1,8-cineole,α-terpineol and limonene were highest in Tian e,and cypressene was in Wangmo.Bacterial Shannon index in Wangmo was 6.3158,higher than other sampling sites.The dominant bacterial phylum in all samples were Proteobacteria,Acidobacteria,Actinobacteria,and Chloroflexi,but it was varied in dominant genera.At the genus level,dominant bacterial genera were mainly affected by pH value and nitrogen content,most genera were positively related with active ingredients.The PICRUST functional prediction results showed that active components were mainly affected by transport and metabolism of bacterial energy and substances,indicating that pH value and nitrogen content in soil could influence the accumulation of fruit medicinal active components by changing rhizophere bacterial diversity.