宁夏地区番茄种质资源核心种质构建策略

Construction strategy of core collections of tomato germplasm resources in Ningxia,China

为筛选出较优的核心种质构建策略,基于前期调查的480份番茄种质资源的20个表型性状数据,依次对8个系统聚类法和5个不同的抽样比例分别进行对比;在此基础上对2个遗传距离、排名前二的抽样比例、3个抽样方法和排名前二的系统聚类法进行组合试验,并对所构建的24个核心种质进行代表性评价。结果表明,系统聚类法的排序为离差平方和法>可变法>最长距离法>可变类平均法>中间距离法>最短距离法>类平均法;抽样比例排序为15%>30%>25%>20%>10%;组合试验最佳的构建方法是:遗传距离为马氏距离,抽样比例为15%,抽样方法为偏离度取样法,系统聚类法为离差平方和法。研究结果为构建核心种质提供了最优构建策略,为宁夏地区番茄种质资源的核心种质构建与相关研究提供了理论依据和技术支持。

In order to select a better strategy for core collection construction.In this study,we based on the data of 20 phenotypic traits of 480 tomato germplasms,8 clustering methods and 5 different sampling proportions were compared;On this basis,two genetic distances,the sampling proportion of the top two,three sampling methods and the clustering method of the top two were used for combination test,and the representative evaluation of the 24 core collections were carried out.The results showed that the order of systematic clustering methods were Ward s method>Weighted pair-group average>Complete linkage>Weighted pair-group average>Centroid method>Single linkage>Unweighted pair-group average.The order of sampling proportions were 15%>30%>25%>20%>10%.The best construction method of combination test was that the genetic distance was Euclidean distance,the sampling proportion was 15%,the sampling method was random sampling method,and the clustering method was the Single linkage.This study provided the optimal construction strategy for the construction of core collection,and provided theoretical basis and technical support for the related research of tomato germplasm resources in Ningxia.