玉米GRMZM2G 455909基因的克隆及其抗病功能初步分析

Cloning of GRMZM2G455909 gene from maize and its functional analysis in transgenic plants

本课题组前期研究获得玉米GRMZM2G 455909基因,该研究拟对GRMZM2G 455909基因开展生物信息学分析,明确其分子特征;构建过表达载体pCAMBIA1301a-GRMZM2G 455909,通过农杆菌介导法获得转GRMZM2G 455909基因拟南芥植株,分析转GRMZM2G 455909基因拟南芥植株抗病能力及其抗病信号通路。研究表明,GRMZM2G 455909基因所编码的蛋白序列属于NBS-LRR类蛋白家族;Pst DC3000处理转GRMZM2G 455909基因拟南芥植株,发现转基因植株体内菌体数量明显下降,其抗病性显著增强,推测Pst DC3000可能诱导转GRMZM2G 45590基因拟南芥体内形成水杨酸抗病信号通路。研究结果可为作物遗传育种抗病性的改良提供新的路径。

The GRMZM2G 455909 gene of maize was discovered in our previous study.In order to determine the molecular characteristics of this gene,bioinformatics analysis and a range of molecular experiments were carried out.The over-expression vector pCAMBIA1301A-GRMZM2G 455909 was constructed and transformed into the Arabidopsis thaliana plants by an Agrobacterium-mediated method,then the disease-resistance and its signal pathway of transgenic Arabidopsis thaliana plants with GRMZM2G 455909 gene were analyzed after inoculation with Pst DC3000.The results showed that the protein encoded by GRMZM2G455909 gene belonged to the NBS-LRR family,and the number of bacterium in leaves from transgenic Arabidopsis thaliana plants with GRMZM2G455909 gene was significantly decreased after inoculation with Pst DC3000 compared with wild-type plants.These suggested that transformation of Arabidopsis thaliana plants with GRMZM2G455909 gene might increase resistance to Pst DC3000 via a SA-mediated signaling pathway.This study could provide a new genetic and breeding approach for improving the disease resistance of crop.