蜂蜜中多酚类化合物的超声辅助双水相提取工艺研究

Ultrasonic-Assisted Aqueous Two-Phase Extraction of Polyphenols in Honey

以荆条蜜为主要原料,采用超声辅助双水相萃取方法提取蜂蜜中多酚类化合物。考察蜂蜜处理液添加量、盐浓度、PEG6000添加量、pH值和超声时间5个因素对蜂蜜中多酚类化合物分配系数和提取率的影响,并在此基础上进行正交试验获得最佳提取工艺条件。结果表明:各因素对蜂蜜多酚提取率的影响排序为盐浓度>PEG6000添加量>超声时间>蜂蜜处理液添加量>pH值。当蜂蜜处理液添加量为0.4 mL、盐浓度为12%、PEG6000添加量为2.5 g、pH值为9.0、超声时间为25 min时,蜂蜜多酚提取率最高,达到94.81%。另外,对荆条蜜样品进行加标回收试验,测得样品中多酚含量为1.246 mg/g、回收率为91.76%~100.26%、标准偏差为0.31%~0.78%。

The ultrasonic-assisted aqueous two-phase extraction(UA-ATPE)was used to extract polyphenols from chaste honey.The influence of the amount of processed honey solution,salt concentration,PEG6000 amount,pH,and ultrasonic time on the partition coefficient and extraction rate of polyphenols in honey samples was investigated,and orthogonal experiment was performed to explore the optimal extraction process.The results showed that the influence of the factors on the extraction rate of honey polyphenols followed the order of salt concentration>PEG6000 amount>ultrasonic time>amount of processed honey solution addition>pH.The extraction rate was the highest(94.81%)under the following conditions:0.4 mL processed honey solution,12%salt,2.50 g PEG6000,pH9.0,and sonication for 25 min.In addition,the standard recovery experiment was conducted,and the results showed that the polyphenol content was 1.246 mg/g and the recovery fell in the range of 91.76%-100.26%,with standard deviations of 0.31%-0.78%.