溴氰虫酰胺对棉蚜和麦长管蚜生物活性的温度效应

Effects of temperature on the bio-activity of cyananthramide to Aphis gossypii Glover and Sitobion avenae Fabricius

【目的】不同温度下,杀虫剂对昆虫的生物活性因杀虫剂品种及昆虫种类的不同而表现出较大的差异。研究杀虫剂的温度效应及其影响机制,为杀虫剂的田间合理使用提供技术支持。【方法】本研究采用浸渍法测定不同温度下双酰胺类杀虫剂溴氰虫酰胺对棉蚜Aphis gossypii Glover和麦长管蚜Sitobion avenae Fabricius的生物活性,并进一步研究亚致死剂量溴氰虫酰胺处理后,棉蚜和麦长管蚜体内重要解毒酶羧酸酯酶(CarE)、谷胱甘肽S-转移酶(GST)、多功能氧化酶(MFO)和UDP-葡萄糖醛酸转移酶(UGT)比活力随温度变化的趋势。【结果】溴氰虫酰胺对棉蚜和麦长管蚜均表现为正温度效应,温度系数最高分别可达+9.44和+8.24。亚致死剂量溴氰虫酰胺作用下,两种蚜虫体内主要解毒酶的比活力测定表明,15℃下,棉蚜体内GST比活力值为211.27 nmol·mg^(﹣1)·min^(﹣1),显著高于对照,30℃下,比活力值为203.29 nmol·mg^(﹣1)·min^(﹣1),显著低于对照。与对照相比,棉蚜GST比活力随温度升高逐渐降低,这一趋势与溴氰虫酰胺对棉蚜的正温度效应的变化一致。同样,麦长管蚜体内GST和UGT比活力较对照的变化趋势也与溴氰虫酰胺对麦长管蚜温度效应的变化相符。【结论】溴氰虫酰胺是对棉蚜和麦长管蚜具有明显的正温度系数药剂,棉蚜体内的GST和麦长管蚜体内的GST和UGT可能参与溴氰虫酰胺对两种蚜虫的温度效应变化过程。

[Objectives]The bio-activity of insecticides to insects varies greatly temperature.Studying the effects of temperature on insecticides,and mechanism underlying these effects,can provide a scientific basis for the rational use of insecticides at different temperatures.[Methods]The dipping method was used to measure the bio-activity of cyantraniliprole,a diamide insecticide,against Aphis gossypii Glover and Sitobion avenae Fabricius at different temperatures.After treatment with a sub-lethal dose of cyantraniliprole,the activity of carboxylesterase(CarE),glutathione S-transferase(GST),multifunctional oxidase(MFO)and UDP-glycosyl transferase(UGT)in A.gossypii and S.avenae was also measured at different temperatures.[Results]Temperature had a positive effect on the bio-activity of cyantraniliprole to A.gossypii and S.avenae;the highest temperature coefficients were+9.44 and+8.24,respectively.After receiving a sub-lethal dose of cyantraniliprole,the specific activity of GST in A.gossypii was 211.27 nmol·mg^(﹣1)·min^(﹣1)at 15℃;significantly higher than in the control.However,at 30℃this fell to 203.29 nmol·mg^(﹣1)·min^(﹣1),which was significantly lower than in the control.Consistent with the positive relationship between temperature and the bio-activity of cyantraniliprole to A.gossypii,GST activity gradually decreased with increasing temperature relative to that in the control.Similarly,differences in GST and UGT activity between treatment and control groups were consistent with those in the bioactivity of cyantraniliprole at different temperatures in S.avenae.[Conclusion]There is a significant,positive correlation between the bioactivity of cyantraniliprole to A.gossypii and S.avenae and temperature.GST and GST may be involved in the effect of temperature on cyantraniliprole in A.gossypii,and UGT may play a similar role in S.avenae.