纤维素酶法提取无患子皂苷的工艺优化

Optimization of enzyme-assisted aqueous extraction technology of sapindus saponins

采用纤维素酶法提取无患子皂苷,探究了不同纤维素酶对不同粒度无患子皂苷提取率的影响,优化了粒度较大的无患子粉末的纤维素酶添加量、酶解时间、酶解温度等提取条件。结果表明,纤维素酶对粒度大于5号筛无患子的皂苷提取率几乎无影响或促进作用较小,大于5号筛粉末可不加纤维素酶提取;1-2号筛无患子粉末酶法提取的较优工艺条件为酶添加量18 U/mL,酶解时间2.5 h,酶解温度50℃;2-3号筛粉末酶法提取的较优工艺条件为酶添加量12 U/mL,酶解时间1.5 h,酶解温度50℃。该方法环保经济,为无患子皂苷工业化提取和原料粒度分级提供了参考。

The sapindus saponins was extracted by enzyme extraction,the effects of different cellulase on the extraction rate of sapo⁃nins from sapindus with different particle size were investigated,and the extraction conditions of sapindus with large particle size in⁃cluding the dose of cellulase,extraction time,extraction temperature were optimized.The results showed that cellulase had a little or no effect on the saponins extraction rate of sapindus powder greater than sieve 5,and those larger than sieve 5 were not extracted with cel⁃lulose.The best process condition for sieve 1-2 particle powder was enzyme addition of 18 U/mL,extraction time of 2.5 h,and extrac⁃tion temperature of 50℃.The best process condition for sieve 2-3 particle powder was enzyme addition of 12 U/mL,extraction time of 1.5 h,and extraction temperature of 50℃.This method was environment-friendly and profitable,which could provide reference for in⁃dustrial extraction of sapindus saponins and the classification of raw material granularity.