二烯丙基一硫拮抗苯诱导大鼠遗传毒性损伤

The protective effects of diallyl sulfide(DAS)on genotoxicity induced by benzene

目的探讨二烯丙基一硫(Diallyl sulfide,DAS)对苯诱导大鼠细胞遗传毒性损伤的保护效果。方法于2018年9月,选取SPF级大鼠60只,适应性喂养5 d后,按体重随机分为6组,对照组、DAS组、苯模型组、苯+DAS低剂量组、苯+DAS中剂量组、苯+DAS高剂量组,每组10只。苯+DAS低中高各剂量组和DAS组分别以40、80、160、160 mg/kg DAS剂量灌胃染毒,对照组和苯模型组给予等体积玉米油。2 h后,苯模型组和苯+DAS各剂量组给予苯(1.3 g/kg)-玉米油混合液(体积分数50%),对照组和DAS组给予等体积玉米油。1次/d,连续4周。收集样品,用于后续指标检测。结果与对照组比较,苯模型组大鼠外周血白细胞计数(WBC)降低65.06%、淋巴细胞比例降低、微核率(‰)增加(P=0.003、0.000、0.000),BMCs中γH2AX平均荧光强度和相对荧光强度分别增加32.69%、32.64%(P=0.001、0.008),PBLs中γH2AX平均荧光强度和相对荧光强度分别增加397.70%、396.26%(P=0.000、0.003);与苯模型组比较,苯+DAS低中高各剂量组大鼠WBC计数均增加(P=0.000、0.003、0.006),微核率(‰)分别降低(P=0.000、0.000、0.000);与苯模型组比较,苯+DAS高剂量组BMCs中γH2AX平均荧光强度和相对荧光强度降低明显(P=0.000、0.000),苯+DAS高剂量组PBLs中γH2AX平均荧光强度和相对荧光强度降低明显(P=0.000、0.000)。结论DAS能有效拮抗苯诱导的大鼠细胞遗传毒性损伤。

Objective To investigate the protective effect of diallyl sulfide(DAS),against benzene-induced genetic damage in rat.Methods In September 2018,Sixty adult male adaptive feeding 5 days,were randomly divided into six groups according to their weight.Control groups,DAS control groups,benzene model groups,benzene+low DAS groups,benzene+middle DAS groups,benzene+High DAS group,10 in each group.Rats in the DAS and DAS control group were orally given DAS at 40,80,160,160 mg/kg,blank control and benzene model groups were given corn oil in the same volume.2 h later,the rats in the benzene model and DAS treatment groups were given gavage administration of benzene(1.3 g/kg)mixed with corn oil(50%,V/V),blank and DAS control groups were given corn oil in the same volume.Once a day,for 4 weeks.Samples were collected for subsequent testing.Results Compared with the blank control group,In benzene treated rat,peripheral WBC count was reduced 65.06%(P=0.003),lymphocyte ratiowas reduced(P=0.000),micronucleus rate was increased(P=0.000),Mean fluorescent intensity and relative fluorescence intensity ofγH2AX in BMCs were increased 32.69%、32.64%(P=0.001、0.008),Mean fluorescent intensity and relative fluorescence intensity ofγH2AX in PBLs were increased 397.70%、396.26%(P=0.000、P=0.003)respectively.Compared with the benzene model group,the WBC count increased respectively(P=0.000、0.003、0.006)and the micronucleus rate decreased(P=0.000、0.000、0.000)in the DAS groups,Mean fluorescent intensity and relative fluorescence intensity ofγH2AX in BMCs were significantly reduced in the high DAS groups(P=0.000、0.000),Mean fluorescent intensity and relative fluorescence intensity ofγH2AX in PBLs were significantly reduced in the low,middle,high DAS groups(P=0.000、0.000).Conclusion DAS can effectively suppress benzene induced genotoxic damage in rats.