摘要
Visualization of the spatiotemporal organization of chromatin is highly desirable in the study of genome function regulations.Clustered regularly interspa-ced short palindromic repeats(CRISPR)/CRISPR-associated endonuclease system has shown great promise for application in real-time chromatin imag-ing due to its DNA targeting ability in living cells.Previous studies typically used fluorescent proteins to generate fluorescent signals which,however,have trade-offs among signal intensity,multiplexibility,and simplicity.
基金
This work was supported by the National Key R&D Program of China(2016YFA0400900)
National Natural Science Foundation of China(21775157,21390414,61475181,and U1632125)
Key Research Program of Frontier Sciences,Chinese Academy of Sciences(QYZDJ-SSW-SLH031).
