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SARS-CoV-2 productively infects primary human immune system cells in vitro and in CoviD-19 patients 被引量:2

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摘要 The severe acute respiratory syndrome coronavirus 2(SARS-CovV-2)infection is associated with a hyperinflammatory state and lymphocytopenia,a hallmark that appears as both signature and prognosis of disease severity outcome.Although cytokine storm and a sustained inflammatory state are commonly associated with immune cell depletion,it is still unclear whether direct SARS-Cov-2 infection of immune cells could also play a role in this scenario by harboring viral replication.We found that monocytes,as well as both B and T lymphocytes,were susceptible to SARS-Cov-2 infection in vitro,accumulating double-stranded RNA consistent with viral RNA replication and ultimately leading to expressive T cell apoptosis.In addition,flow cytometry and immunofluorescence analysis revealed that SARS-Cov-2 was frequently detected in monocytes and B lymphocytes from coronavirus disease 2019(CoVID-19)patients.The rates of SARS-Cov-2-infected monocytes in peripheral blood mononuclear cells from CoVID-19 patients increased over time from symptom onset,with SARS-CoV-2-positive monocytes,B cells,and CD4+T lymphocytes also detected in postmortem lung tissue.These results indicated that SARS-CoV-2 infection of blood-circulating leukocytes in covID-19 patients might have important implications for disease pathogenesis and progression,immune dysfunction,and virus spread within the host.
出处 《Journal of Molecular Cell Biology》 SCIE CAS CSCD 2022年第4期51-64,共14页 分子细胞生物学报(英文版)
基金 supported by Conselho Nacional de Desenvolviment Coientificoe Tecnologico(CNPq 310100/2017-8,403201/2020-9,and INCT 465539/2014-9) Fundagaode Amparoa Pesquisado Estado deSao Paulo(FAPESP 2013/16349-2 and 2014/02438-6) the National Institutes of Health(NIH Al163019).M.C.P and R.G.were funded by CNPq(380849/2020-8).
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