脑内三磷酸腺苷的光电化学活体分析

In Vivo Photoelectrochemical Analysis of ATP in the Brain

神经化学信号传递是实现大脑复杂功能的基础,因此发展神经化学信号的活体原位检测方法,对于探索脑功能和脑疾病的神经化学分子机制具有重要意义。光电化学传感技术具有灵敏度高、背景信号低和易于微型化等优点,是活体原位分析的潜在有力工具。然而,常见的光电活性材料需要短波长的光激发,其组织穿透深度不足,限制了在活体分析中的应用。基于此,本文构建了一种可近红外激发的光电化学微传感器,用于脑内三磷酸腺苷(ATP)的原位检测。将稀土掺杂的上转换纳米颗粒(UCNPs)引入传感界面,用UCNPs的发光激发电极表面的光电活体材料产生光电流信号,通过荧光染料(TAMRA)标记的核酸适配体调节UCNPs的发光,发展一种基于光学调控策略检测脑内ATP的光电化学传感新方法。所制备的微传感器成功用于炎症模型中小鼠脑内ATP的原位检测,初步探索了脑部炎症与ATP水平变化的关系。

Neurochemical signal transmission is the basis of complex brain functions, so the development of in vivo in situ detection methods of neurochemical signals is of great significance for us to explore the neurochemical molecular mechanisms of brain functions and brain diseases.Photoelectrochemical sensing has the advantages of high sensitivity, low background signal and easy miniaturization, and is a potential powerful tool for in vivo analysis.However, common photoactive materials require short-wavelength light excitation, which tissue penetration depth is insufficient, limiting the application of in vivo analysis.This study constructed a near-infrared excited photoelectrochemical microsensor for in vivo detection of ATP in the brain.Lanthanide doped upconversion nanoparticles(UCNPs) have been introduced into the sensing interface, where its upconversion luminescence could excited the photoactive materials to produce photocurrent response.In addition, fluorescent dye(TAMRA)-labeled ATP aptamers was used to modulated the luminescence of UCNPs, which in turn modulated the photocurrent.Based on this, a novel photoelectrochemical sensing method for detecting ATP in brain has been developed.The microsensor was successfully used for in vivo detection of ATP in the brain of mice with inflammation model, and the relationship between brain inflammation and ATP level was preliminarily explored.