分子印迹包裹的碳量子点荧光传感器特异性吸附检测核黄素

Molecularly Imprinted Carbon Quantum Dots Fluorescent Sensor for Specific Adsorption and Detection of Riboflavin

利用核黄素作为模板分子印迹在溶胶凝胶分子层并包裹碳量子点,制备荧光传感器(Carbon Quantum Dots@Molecular Imprinted Polymers,CD_(S)@MIPs)。在激发波长为370 nm时,该传感器特异性吸附核黄素后,520 nm处荧光随核黄素浓度的变化而变化,即520 nm处的荧光作为变量信号,碳量子点在460 nm的荧光作为参考信号,形成比率荧光传感器。核黄素的浓度与I_(520)/I_(460)的荧光比值呈现线性相关关系,线性范围为0.15~7.0μmol/L,检出限为8.48 nmol/L。相比于直接荧光检测核黄素的方法,此方法具有特异性、抗背景干扰性等优点,可应用于检测果汁中的核黄素。

The carbon quantum dots(Carbon Quantum Dots@Molecular Imprinted Polymers,CDs@MIPs)fluorescent sensor was formed by using riboflavin as a template molecule to imprint in the sol-gel molecular layer and encapsulate in the carbon quantum dots.When the excitation wavelength is 370 nm,the fluorescence at 520 nm changed with the change of riboflavin concentration,after the sensor specifically adsorbed riboflavin,the fluorescence at 520 nm is used as the variable signal,and the fluorescence of carbon quantum dots at 460 nm is used as the reference signal,forming a ratio fluorescent sensor.The concentration of riboflavin has a linear correlation with the fluorescence ratio of I_(520)/I_(460).The linear range is 0.15-7.0μmol/L,and the detection limit is 8.48 nmol/L.Compared with the method for direct fluorescence detection of riboflavin,this method has the advantages of good specificity and anti-background interference,and can be applied to the detection of riboflavin in fruit juice.