雪莲黄酮胶囊对高原缺氧诱导小鼠心肌损伤的保护作用研究

Protective effect of Saussurea involucrata flavone capsule on myocardial injury induced by hypoxia at high altitude in mice

目的研究雪莲黄酮胶囊对模拟高原缺氧环境小鼠心肌组织的保护作用及其机制。方法将64只Balb/c小鼠随机分为正常对照组、缺氧模型组、阳性对照组和雪莲黄酮胶囊组,以500 mg/kg剂量进行单次灌胃给药后,在模拟海拔8000 m环境停留9 h,对心肌组织进行显微与超微结构观察,并检测缺氧相关指标的变化。通过RT-PCR法检测缺氧相关基因转录水平的变化,并通过蛋白质印迹法检测缺氧相关基因蛋白表达的变化,研究雪莲黄酮胶囊的作用机制。结果雪莲黄酮胶囊可明显缓解模拟高原缺氧环境对小鼠心肌的病理学损伤,提高心肌组织T-AOC活力,降低LD累积,还可以降低心肌组织HIF-1α、VEGF mRNA的表达水平,提高SOD、CAT mRNA及蛋白的表达水平。结论雪莲黄酮胶囊具有良好的抗高原缺氧作用,可保护心肌组织结构和功能,调节能量代谢和提高抗氧化能力。其机制可能与提高高原缺氧小鼠抗氧化能力、调节能量代谢以及影响HIF-1α、VEGF、SOD、CAT mRNA的蛋白表达有关。

Objective To study the protective effect and mechanism of Saussurea involucrata flavone capsule on the myocardial tissue of mice in simulated plateau hypoxia environment.Methods 64 Balb/c mice were randomly divided into normal control group,hypoxia model group,positive control group and Xuelian flavonoid capsule group.Myocardial tissue was observed microscopically and ultra-structurally,and the changes of hypoxia-related indexes were detected.The changes in the transcription levels of hypoxia-related genes were detected by RT-PCR,and the changes in the protein expressions of hypoxia-related genes were detected by Western blotting to study the mechanism of action of Xuelian flavone capsules.Results Saussurea involucrata flavone capsule had significantly alleviated the pathological damage to the myocardium of mice caused by simulated altitude hypoxia,increased the activity of T-AOC in myocardial tissue,reduced the accumulation of LD,and also reduced the expression levels of HIF-1αand VEGF mRNA in myocardial tissue,increased the SOD,CAT mRNA and protein expression levels.Conclusion Saussurea involucrata flavone capsule have good anti-altitude hypoxia effect,which could protect myocardial tissue structure and function,regulate energy metabolism,and improve antioxidant capacity.The mechanism might be related to improving the antioxidant capacity,regulating energy metabolism,and affecting the protein expression of HIF-1α,VEGF,SOD and CAT mRNA in high altitude hypoxic mice.