基于UHPLC-Q-TOF/MS技术鉴定肺脾气虚汤的化学成分及其入血成分

Identification of chemical constituents and blood-absorbed ingredients of FeiPi QiXu decoction by UHPLC-Q-TOF/MS

目的对肺脾气虚汤中的主要化学成分及SD大鼠口服后的入血成分进行鉴定,为分析其药效物质基础提供参考。方法采用超高效液相色谱-四级杆飞行时间串联质谱(UHPLC-Q-TOF/MS)技术对肺脾气虚汤提取液及SD大鼠口服肺脾气虚汤后的血清样本进行数据采集,并通过对照品、自建质谱数据库、MS FINDER数据库及查阅文献对其化学成分及入血成分进行快速鉴定。结果共从肺脾气虚汤中鉴定出56种化学成分,主要为黄酮类、三萜类、酚类成分,化合物大多来源于炙黄芪、陈皮、茯苓和党参;含药血清中检测到11种原形成分,包含芒柄花素、黄芪甲苷IV和新橙皮苷等化合物。结论该研究较为全面的阐明了肺脾气虚汤化学成分组成及药材来源,并初步分析了其入血成分,为进一步对该方的药效物质基础及质量控制研究提供了科学依据。

Objective To identify the chemical constituents and blood-absorbed ingredients of FeiPi QiXu decoction,and provide reference for clarifying the material basis of its efficacy.Methods An ultrahigh performance liquid chromatography-quadrupole-time of flight-mass spectrometry(UHPLC-Q-TOF/MS)method was applied to analyze the data of FeiPi QiXu decoction extraction and the serum samples of SD rats after oral administration.The chemical constituents and blood-absorbed ingredients were quickly identified by chemical reference substance,self-built mass spectrometry database,MSFINDER database and the relevant literature.Results A total of 56 chemical constituents were identified from Feiqi Deficiency Decoction,mainly were flavonoids,triterpenoids and phenols.Most of the compounds came from Radix Astragali praeparata cum melle,Citri Reticulatae Pericarpium,Poria and Codonopsis Radix.11 prototype compounds were identified in the serum,including Formononetin,Astragaloside IV and Neohesperidin.Conclusion This study comprehensively clarified the chemical composition and source of medicinal materials,and preliminarily analyzed its blood-absorbed ingredients,which could provide a scientific basis for further study on the pharmacodynamic material basis and quality control of FeiPi QiXu decoction.