41份木豆萌发期耐盐性综合评价

Evaluation of salt tolerance in 41 pigeon pea varieties during germination

对41份木豆(Cajanus cajan)种质资源进行萌发期耐盐性综合评价,测定发芽率、发芽指数、活力指数、根系表面积和根系体积等10项指标,通过变异系数分析、相关性分析、主成分分析、隶属函数分析和聚类分析,对其进行萌发期耐盐性综合评价。同时,对筛选出的不同耐盐性木豆种质,测定幼苗的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,分析盐胁迫下不同耐盐性木豆的生理响应机理的差异。结果表明:41份木豆种子萌发期耐盐性存在显著差异,筛选出相对根长、相对根冠比、相对发芽指数和相对根系平均直径可作为木豆萌发期耐盐性评价指标。基于隶属函数值进行聚类分析,可将41份木豆种质资源划分成高耐盐型(1份)、耐盐型(10份)、中间型(23份)和盐敏感型(7份) 4个类别。不同耐盐性木豆在盐胁迫下的生理响应表明,高耐盐型和耐盐型材料的细胞质膜透性和酶活能力优于盐敏感型。

In this study, we identified the salt tolerance of 41 pigeon pea(Cajanus cajan) germplasm varieties during the germination period. Furthermore, we identified 10 physiological response indicators, including germination rate, germination index, vigor index, root surface area, and root volume. Principal component, membership-function, and cluster analyses were used to comprehensively evaluate salt tolerance during the germination period. Concurrently, the superoxide dismutase(SOD), peroxidase(POD), and catalase(CAT) activities in the seedlings were determined, and the physiological response mechanisms of different salt-tolerant pigeon pea seedlings under salt stress were analyzed. The results revealed significant differences in the salt tolerance of 41 pigeon pea germplasm varieties during germination, with relative root length, crown ratio, germination index, and average diameter being selected as the key indices of germination salt tolerance. Cluster analysis based on membership-function values divided the 41 pigeon pea germplasm varieties into four categories, namely highly salt tolerant(1 variety), salt tolerant(10 varieties), intermediate tolerance(23 varieties), and salt-sensitive(7 varieties). Ultimately, using pigeon peas that exhibit varying levels of salt tolerance, we reveal that the cytoplasmic membrane permeability and enzyme viability of high-salt-resistant and salt-resistant varieties generate superior physiological responses compared to salt-sensitive varieties.