外膜蛋白OmpR对副溶血弧菌致病特性的影响

Influence of outer membrane protein OmpR on pathogenicity of Vibrio parahaemolyticus

【目的】探究OmpR在副溶血弧菌生物学特性和致病性中发挥的作用。【方法】利用同源重组技术构建了副溶血弧菌ompR基因缺失株(ΔompR)和互补株(CΔompR),分析各菌株的生长特性、运动性和生物被膜形成能力的差异;比较各菌株对细胞黏附、细胞毒性和小鼠致病性的影响。【结果】ompR基因缺失对副溶血弧菌的生长特性、运动性以及细胞毒性无显著影响。但与野生株相比,ΔompR的生物被膜的形成能力显著降低;感染ΔompR的小鼠存活率升高了25%,病变程度更低;ΔompR在小鼠心脏、肝脏和肾脏中的载菌量显著低于野生株,互补株毒力基本恢复至野生株水平。【结论】OmpR参与副溶血弧菌生物被膜形成和致病过程,是副溶血弧菌潜在的毒力因子。

[Objective] To study the role of OmpR in biological characteristics and pathogenicity of Vibrio parahaemolyticus. [Methods] The ompR gene deletion mutant(ΔompR) and complementary strain(CΔompR) of Vibrio parahaemolyticus were constructed using homologous recombination. The differences of growth characteristics, motility and biofilm formation ability of each strain were analyzed. Furthermore, the effects of various strains on cell adhesion, cytotoxicity and mouse pathogenicity were compared. [Results] The ompR deletion had no significant effect on the growth characteristics, motility and cytotoxicity of V. parahaemolyticus. However, compared with the wild-type(WT) strain, ΔompR showed markedly reduced biofilm formation ability. Mice infected with ΔompR had a 25% higher survival rate and fewer lesions. The bacterial load of ΔompR in mouse heart,liver and kidney was significantly lower than that of the WT strain, whereas the complementary strain restored the virulence to the WT level. [Conclusion] OmpR is involved in the pathogenic process in mice. It is a potential virulence factor of V. parahaemolyticus.