芥蓝TCP家族全基因组鉴定及表达分析

Genome identification and expression analysis of TCPfamily in Chinese kale

为明确芥蓝TCP家族相关基因在叶片发育中的功能,基于甘蓝基因组数据,分析鉴定出芥蓝TCP基因家族有40个成员,参考拟南芥同源TCP基因注释及拟南芥数据库基因的相对表达量,初步选取BoTCP21和BoTCP25基因,采用qRT-PCR分析。结果表明:BoTCP21和BoTCP25均在叶片中大量表达,但二者在真叶不同部位的表达模式存在差异。为了进一明确TCP基因家族中参与叶片发育的基因,采用生物信息学方法并结合qRT-PCR对BoTCP家族进行了全面分析。结果显示:40个BoTCP都属于非分泌亲水性蛋白,分别定位在8条染色体上;系统进化树构建和亲缘关系分析将芥蓝中的BoTCP成员归为3类,其中PCF亚家族有19个成员,CYC亚家族8个成员,CIN亚家族13个成员。qRT-PCR结果表明:BoTCP21和BoTCP25在真叶和薹叶中具有较高的表达量,BoTCP14在开花结荚的植株根部表达量较高,而BoTCP16则在抽薹植株的根部表达量最高,说明BoTCP家族成员在组织部位表达存在时空特异性且广泛参与了植株的形态建成和器官发育。

In order to clarify the function of TCPfamily related genes in Chinese kale in leaf development,based on cabbage genome data,this study identified 40members of TCPgene family in Chinese kale.Referring to the annotation of homologous TCP genes in Arabidopsis and the relative expression of genes in Arabidopsis database,BoTCP21 and BoTCP25 genes were preliminarily selected and analyzed by qRT-PCR experiment.The results showed that BoTCP21and BoTCP25 were widely expressed in leaves,but their expression patterns were different in different parts of true leaves.In order to find out the genes involved in leaf development in TCP gene family,the BoTCP family was comprehensively analyzed by bioinformatics and qRT-PCR experiment.The results showed that 40 BoTCP were nonsecreted hydrophilic proteins,which were located on 8chromosomes respectively.The members of BoTCPin Chinese kale are classified into three categories by phylogenetic tree construction and phylogenetic analysis,among which there are nineteen members in PCF subfamily,eight members in CYCsubfamily and thirteen members in CINsubfamily.The results of qRT-PCR showed that BoTCP21and BoTCP25had higher expression levels in true leaves and bolting leaves,BoTCP14had higher expression levels in the roots of flowering and podding plants,while BoTCP16 had the highest expression levels in the roots of bolting plants,which indicated that the expression of BoTCPfamily members in the tissue parts was spatio-temporal specific and widely participated in the morphogenesis and organ development of plants.