摘要
目的探讨单相和双相抑郁患者外周血单核细胞内糖原合成酶激酶-3β(glycogen synthase kinase-3 GSK-3β)蛋白表达差异。方法从门诊及病房患者中选取单相及双相抑郁障碍患者各30例。正常对照30例。入组当天收集受试者一般资料,并评定单相及双相抑郁组汉密顿抑郁量表(hamilton depression scale HAMD)评分,取受试者静脉血并提取其外周血单核细胞。利用免疫印迹法检测其细胞中总糖原合成酶激酶-3β(total glycogen synthase kinase-3beta总GSK-3β)及磷酸化GSK-3β(phosphorylated-glycogen synthase kinase-3beta p-GSK-3β)蛋白含量。结果(1).单相与双相抑郁障碍组患者p-GSK-3β/总GSK-3β比值均较正常组增加,差异有统计学意义(P=0.000);双相较单相抑郁障碍组p-GSK-3β/总GSK-3β比值增加,差异有统计学意义(P=0.000)。(2).双相抑郁障碍患者p-GSK-3β/总GSK-3β比值与其HAMD分数呈正相关(R=0.424,P=0.019)。结论p-GSK-3β/总GSK-3β比值变化可能在单相及双相抑郁障碍病理机制中发挥重要作用,也可能在早期鉴别单相和双相抑郁障碍发挥其作用。
Objective To investigate there is differential expression of glycogen synthase kinase-3beta(GSK-3β)in peripheral blood between unipolar and bipolar depression.Methods The outpatient and ward patients were selected,including 30 patients of bipolar depression and 30 patients of unipolar depression.And there were 30 cases in the normal group.All participant general states were collected under informed consent.Unipolar and bipolar depression groups also were all evaluated by hamilton depressive scale(HAMD).All participants’20ml peripheral venous blood was collected.The proteins of total glycogen synthase kinase-3beta(total GSK-3β)and phosphorylated-glycogen synthase kinase-3beta(p-GSK-3β)in peripheral blood mononuclear cells were measure by Western blotting.Result(1).The ratio of p-GSK-3βto total GSK-3βbetween the unipolar depression group and bipolar depression group are higher than the normal group,and there was a significant difference(P=0.000).The ratio of p-GSK-3βto total GSK-3βin the bipolar depression group was higher than the unipolar depression group,and there was significant difference(P=0.000).(2).The ratio of p-GSK-3βto total GSK-3βin the bipolar depression group was positively correlated with the their scores of HAMD(r=0.424,P=0.019).Conclusion The changes of the ratio of p-GSK-3βto total GSK-3βmay involve in the pathogenesis of unipolar and bipolar depression;also may play a key role in the differential diagnosis between unipolar and bipolar depression.
作者
刘芊
王学滨
刘宏
刘卫平
张玉坤
姜飞
LIU Qian;WANG Xuebin;LIU Hong(Dalian Seventh People’s Hospital,Dalian 116023,China)
出处
《国际精神病学杂志》
2022年第4期605-608,共4页
Journal Of International Psychiatry
基金
大连市医学科学研究计划项目(编号:1911049)。