SF3B1、UBE2V2和SETD2的表达与老年骨质疏松性椎体骨折的相关性研究

Expression of SF3B1, UBE2V2 and SETD2 and association with osteoporotic vertebral fractures inelderly patients

目的探究剪切因子3B亚基1(subunit 1 of splicing factor 3b,SF3B1)、泛素结合酶E2变体2(ubiquitin conjugating enzyme E2 V2,UBE2V2)和组蛋白甲基转移酶(SET domain-containing protein 2,SETD2)的表达与老年骨质疏松性椎体骨折(osteoporotic vertebral fracture,OVF)的相关性。方法收集2020年8月至2021年8月烟台市烟台山医院31例骨质疏松性椎体骨折(vertebral fracture,VF)(VF组)和16例骨质疏松非椎体骨折(non vertebral fracture,NVF)(NVF组)的老年患者(年龄>60岁)的外周血样本,提取RNA进行转录组测序筛选差异表达基因。通过基因本体论(gene ontology,GO)分析、蛋白相互作用(protein protein interaction,PPI)网络分析、ROC曲线分析筛选VF相关基因。采用qRT-PCR检测基因的表达水平。结果相对于NVF组,VF中有822个差异表达基因,691个上调,131个下调。qRT-PCR结果显示,相对于NVF患者(1.55±0.33)(1.70±0.33)(1.64±0.33),VF患者外周血中SF3B1(1.83±0.23)(t=2.84,P=0.008)、UBE2V2(2.24±0.43)(t=3.91,P<0.001)的表达增强,SETD2(1.18±0.46)(t=3.25,P=0.003)表达降低。ROC曲线分析显示,SF3B1、UBE2V2和SETD2在VF中的AUC分别为0.8034(P=0.007)、0.8145(P=0.005)和0.7863(P=0.0014)。结论SF3B1、UBE2V2和SETD2与老年OVF高度相关,对OVF的诊断和预测具有重要价值。

Objective To explore the relationship between the expression of SF3B1,UBE2V2,SETD2 and osteoporotic vertebral fracture(OVF)in elderly patients.Methods Peripheral blood samples were collected from 31 elderly patients with osteoporotic vertebral fractures(VF group)and 16 elderly patients with osteoporotic non-vertebral fractures(NVF group)in Yantai Mountain Hospital.RNA was extracted for transcriptome sequencing to screen for differentially expressed genes.VF related genes were screened by Gene Ontology(GO)analysis,protein protein interaction(PPI)network analysis and ROC curve analysis.Qrt-pcr was used to detect gene expression levels.Results Compared with NVF group,691 genes were up-regulated while 131 genes were down regulatedin VF group.qRT-PCR results revealed that,compared with NVF patients(1.55±0.33)(1.70±0.33)(1.64±0.33),SF3B1(1.83±0.23)(t=2.84,P=0.008),UBE2V2(2.24±0.43)(t=3.91,P<0.001)expression were increased while SETD2(1.18±0.46)(t=3.25,P=0.003)expression was decreased in peripheral blood of VF patients.ROC curve analysis showed that the AUCs of SF3B1,UBE2V2 and SETD2 in VF were 0.8034(P=0.007),0.8145(P=0.005)and 0.7863(P=0.0014),respectively.Conclusion SF3B1,UBE2V2 and SETD2 are highly correlated with OVF in elderly patients,and are of great value in the diagnosis and prediction of OVF.