GGDEF结构域在c-di-GMP代谢中的作用及其结构基础

The Role of the GGDEF Domain in C-di-GMP Metabolism and Its Structural Basis

环二鸟苷酸(cyclic diguanylate monophosphate,c-di-GMP)是一种重要的第二信使,可调节细菌的各种生命活动,包括生物膜形成、细胞分化、菌体运动和毒力因子产生等。在c-di-GMP代谢过程中,GGDEF结构域发挥重要作用。一方面,GGDEF结构域广泛存在于二鸟苷酸环化酶(diguanylate cyclases,DGCs)上,催化c-di-GMP的合成;另一方面,GGDEF结构域也可与EAL等结构域串联,参与c-di-GMP的分解。该文系统介绍了c-di-GMP的代谢过程及其生理作用,重点论述了GGDEF结构域有效调控c-di-GMP代谢的结构基础和机制,并讨论了该领域研究的应用价值与未来方向。

C-di-GMP (cyclic diguanylate monophosphate) is an important second messenger in bacteria which can regulate biological movements,including biofilm formation,cell differentiation,bacterial motility and virulence factor production.The GGDEF domain plays an important role in c-di-GMP metabolism.On the one hand,GGDEF domain widely exists in DGCs (diguanylate cyclases),catalyzing the synthesis of c-di-GMP.On the other hand,GGDEF domain can also be connected with EAL domain and other domains to participate in c-di-GMP degradation.This paper systematically introduces the metabolic process and physiological action of c-di-GMP,focuses on the structural basis and mechanism of GGDEF domain in effectively regulating c-di-GMP metabolism,and discusses the application value and future direction of research in this field.