STIL和PRR11在宫颈癌组织的表达水平及其与淋巴结转移的相关性

The Expression Levels of STIL and PRR11 in Cervical Cancer Tissues and Their Correlation with Lymph Node Tissue Metastasis

目的:分析SCL/TALl断裂点(STIL)和富含脯氨酸蛋白11(PRR11)在宫颈癌组织的表达水平及其与淋巴结转移的相关性。方法:选择我院2020年1月至2022年1月收治的143例宫颈癌患者作为研究对象,术中收集患者宫颈癌组织和肿瘤邻近正常癌旁组织。采用蛋白质印迹法(Western Blot)对癌组织和癌旁组织中STIL和PRR11蛋白水平进行检测;采用实时荧光定量PCR(qRT-PCR)对STIL和PRR11 mRNA水平进行检测;比较不同FIGO分期患者中STIL和PRR11蛋白水平;比较淋巴结转移和非转移患者中STIL和PRR11蛋白水平;采用受试者工作特征(ROC)曲线分析STIL和PRR11水平对宫颈癌淋巴结转移的诊断价值,曲线下面积(AUC)表示诊断价值;采用Pearson分析STIL和PRR11蛋白表达之间的相关性。结果:宫颈癌组织中STIL、PRR11蛋白水平及其基因相对表达量均明显高于癌旁组织(P<0.05);FIGO分期越高,STIL、PRR11蛋白水平明显越高(P<0.05);淋巴结转移患者中STIL、PRR11蛋白水平均明显高于未转移(P<0.05);STIL蛋白、PRR11蛋白诊断宫颈癌淋巴结转移的AUC分为0.733、0.729(P<0.05)。Pearson相关性分析显示,癌组织STIL和PRR11蛋白表达具有显著的相关性(P<0.05)。结论:STIL、PRR11在宫颈癌组织中呈现高表达,其表达量升高暗示宫颈癌患者出现淋巴结转移风险增加,可以评估宫颈癌淋巴结转移。

Objective:To analyze the expression levels of SCL/TALl interrupting locus(STIL)and proline-rich protein 11(PRR11)in cervical cancer tissues and their correlation with lymph node metastasis.Methods:A total of 143 patients with cervical cancer admitted to the hospital were enrolled as the research subjects between January 2020 and January 2022.During surgery,cervical cancer tissues and adjacent normal para-carcinoma tissues were collected.The levels of STIL and PRR11 in cancer tissues and para-carcinoma tissues were detected by Western Blot.The mRNA levels of STIL and PRR11 were detected by quantitative real-time polymerase chain reaction(qRT-PCR).The levels of STIL and PRR11 in patients with different FIGO staging,and between patients with or without lymph node metastasis were compared.The diagnostic value of STIL and PRR11 for lymph node metastasis was analyzed by receiver operating characteristic(ROC)curves,which were represented by the area under the curve(AUC).The correlation between STIL and PRR11 was analyzed by the Pearson method.Results:The levels of STIL and PRR11,and relative expression levels of their genes in cervical cancer tissues were significantly higher than those in para-carcinoma tissues(P<0.05).The higher the FIGO staging,the higher the levels of STIL and PRR11(P<0.05).The levels of STIL and PRR11 in patients with lymph node metastasis were significantly higher than those without metastasis(P<0.05).AUC values of STIL and PRR11 in the diagnosis of lymph node metastasis were 0.733 and 0.729,respectively(P<0.05).Pearson correlation analysis showed that there was a significant correlation between STIL and PRR11 in cancer tissues(P<0.05).Conclusion:The expressions of STIL and PRR11 are up-regulated in cervical cancer tissues.And their increase indicates the increased risk of lymph node metastasis.STIL and PRR11 can assess lymph node metastasis in cervical cancer.