血浆丝氨酸蛋白酶抑制剂E1蛋白水平与幽门螺杆菌感染的关系研究

Identification of Relationship between Helicobacter Pylori Infection and Serum SERPIN E1 Protein Level in Adults

目的:探讨人体血浆丝氨酸蛋白酶抑制剂E1(serpin peptidase inhibitor clade E member 1,SERPINE1)蛋白水平与幽门螺杆菌(Helicobacter pylori,Hp)感染的关系,为Hp相关性胃癌的机制研究提供理论依据。方法:纳入417名受试者,分为Hp阴性组(192例)以及Hp阳性组(225例),检测受试者血浆生化指标及SERPINE1蛋白水平。结果:Hp阴性组、Hp阳性组中受试者血浆SERPINE1蛋白水平分别为5.38(4.56~6.18)(ng/mL)、8.20(6.91~9.34)(ng/mL),差异具有统计学意义(P<0.01);依据血浆SERPINE1蛋白水平的四分位数间距对受试者进行分组,相关性分析显示随着血浆SERPINE1蛋白水平的升高,Hp感染率也逐步升高(P<0.001);血浆SERPINE1蛋白水平构建诊断Hp感染的受试者工作曲线(Receive operating characteristic curve,ROC),其曲线下面积为0.906,且最佳截点值为6.49ng/mL,诊断Hp感染的灵敏度为84.4%,特异度为83.3%。结论:血浆SERPINE1蛋白水平与Hp感染具有一定的相关性,SERPINE1蛋白可能在Hp相关性胃癌发生发展中具有重要作用。

Objective:To identify the relationship between Helicobacter pylori(Hp)infection and serum SERPIN E1 protein level in adults and offer a theoretical basis for investigating the mechanisms by which Hp induces gastric cancer.Methods:A total of 417 subjects were allocated to the Hp-negative group(n=192)or the Hp-positive group(n=225).All participants had fully detailed records of biochemical data and results of serum SERPIN E1 testing.Results:Serum SERPIN E1 level was significantly different(P<0.01)between the Hp-negative group and Hp-positive group:5.38(4.56~6.18)(ng/ml))versus 8.20(6.91~9.34)(ng/ml).All the participants were divided into 4 groups by quartile values of serum SERPIN E1 and a linear correlation between the prevalence of Hp infection and quartiles of serum SERPIN E1 levels was observed(P<0.001).Based on the receiver operating characteristic curve(ROC)for predicting Hp infection in participants,the serum level of SERPIN E1 with optimal sensitivity(84.4%)and specificity(83.3%)was about 6.49ng/ml and the area under the ROC curve was 0.906.Conclusion:Serum level of SERPINE1 is closely associated with Hp infection in adults.SERPIN E1 may play an important role in the pathogenesis of Hp-induced gastric cancer.