MMP-2、ADAM17在骨肉瘤组织中的表达及其临床意义

Expression of MMP-2 and ADAM17 in Osteosarcoma and its Clinical Significance

【目的】探讨基质金属蛋白酶2(MMP-2)及整合素-金属蛋白酶17(ADAM17)在骨肉瘤组织中的表达水平及其临床意义。【方法】收集本院骨科开放性手术中所切除的53例骨肉瘤组织及癌旁正常组织(距肿瘤边缘至少2 cm),检测并比较骨肉瘤组织及癌旁正常组织中的MMP-2及ADAM17的表达水平,并分析两者的相关性及其与骨肉瘤患者临床病理特征的关系。【结果】MMP-2在骨肉瘤组织中的阳性表达率为73.58%,显著高于癌旁正常组织的18.87%,ADAM17在骨肉瘤组织中的阳性表达率为79.25%,显著高于癌旁正常组织的5.66%,其差异均有统计学意义(P<0.05);MMP-2、ADAM17蛋白在骨肉瘤组织中的表达水平显著高于癌旁正常组织,差异有统计学意义(P<0.05);MMP-2、ADAM17的阳性表达率与肿瘤是否有远处转移、Ennecking分期有关(P<0.05),与患者年龄、性别及肿瘤直径无关(P>0.05)。Spearman相关性结果显示:MMP-2与ADAM17在骨肉瘤组织中的表达呈正相关(P<0.05)。【结论】MMP-2、ADAM17在骨肉瘤组中的阳性表达率显著高于癌旁正常组织,其表达与肿瘤是否远处转移及Ennecking分期有关,且骨肉瘤组织中MMP-2表达与ADAM17表达呈正相关,对于骨肉瘤的诊断、预后及治疗具有重要意义。

【Objective】To investigate the expression of matrix metalloproteinase 2(MMP-2)and a disintegrin and metalloproteinase 17(ADAM17)in osteosarcoma and its clinical significance.【Methods】The osteosarcoma tissues and normal adjacent tissues(at least 2 cm from the tumor edge)were collected from 53 cases of orthopaedic open surgery in our hospital.The expression levels of MMP-2 and ADAM17 in 53 osteosarcoma tissues were detected and compared,and to analyze the correlation between them and the clinicopathological characteristics of osteosarcoma patients.【Results】The positive expression rate of MMP-2 in osteosarcoma was 73.58%,which was significantly higher than 18.87%in normal tissues adjacent to cancer.The positive expression rate of ADAM17 in osteosarcoma was 79.25%,which was significantly higher than 5.66%in normal tissues adjacent to cancer(P<0.05);The expression level of MMP-2 and ADAM17 protein in osteosarcoma was significantly higher than that in normal tissues(P<0.05);The positive expression rates of MMP-2 and ADAM17 were correlated with distant metastasis and ennecking stage(P<0.05),but not with age,sex and tumor diameter(P>0.05).Spearman correlation showed that MMP-2 was positively correlated with ADAM17 expression in osteosarcoma(P<0.05).【Conclusion】The positive expression rate of MMP-2 and ADAM17 in osteosarcoma group is significantly higher than that in adjacent normal tissues.The expression of MMP-2 and ADAM17 is related to the distant metastasis and ennecking stage of the patients.The expression of MMP-2 and ADAM17 in osteosarcoma is positively correlated,which is of great significance for the diagnosis,prognosis and treatment of osteosarcoma.