miR-130a、RUNX3在食管鳞癌中表达的相关性及其临床意义

Correlation and Clinical Significance of miR-130a and RUNX3 Expression in Esophageal Squamous Cell Carcinoma

【目的】探讨miR-130a、重组人Runt相关转录因子3(RUNX3)在食管鳞癌中表达的相关性及其临床意义。【方法】本院接受手术治疗的84例食管鳞癌患者,取患者手术时癌组织为研究对象,相应的癌旁组织为对照。采用qRT-PCR检测组织中miR-130a表达水平;采用Westernblot法及免疫组化法检测组织中RUNX3蛋白表达。对所有患者进行随访,探讨miR-130a与RUNX3表达对食管鳞癌患者预后的影响。【结果】与癌旁组织相比,食管鳞癌组织中miR-130a表达水平较高(P<0.05),RUNX3蛋白表达水平较低(P<0.05)。食管鳞癌组织中miR-130a与RUNX3蛋白表达水平呈负相关(P<0.05)。miR-130a和RUNX3蛋白表达水平与T分期、组织分化程度、淋巴结转移显著相关(P<0.05)。Kaplan-Meier生存曲线分析显示,miR-130a高表达患者中位生存时间显著短于miR-130a低表达患者(P<0.05);RUNX3低表达患者中位生存时间显著短于RUNX3高表达患者(P<0.05)。多因素Cox分析表明,T分期T3~T4期、有淋巴转移、miR-130a高表达、RUNX3低表达是食管鳞癌患者预后不良的独立危险因素(P<0.05)。【结论】食管鳞癌癌组织中miR-130a表达水平上调、RUNX3蛋白表达水平下调,二者与食管鳞癌患者预后不良密切相关,且miR-130a高表达、RUNX3低表达的食管鳞癌患者发生预后不良的风险较大。

【Objective】To investigate the correlation and clinical significance of the expression of miR-130a and recombinant human Runt-related transcription factor 3(RUNX3)in esophageal squamous cell carcinoma.【Methods】A total of 84 patients with esophageal squamous cell carcinoma who were treated in our hospital were collected.The cancer tissues and the corresponding adjacent tissues of patients at the time of surgery were taken for exam.The qRT-PCR was performed to detect the expression level of miR-130a in tissues;Western blot and immunohistochemical methods were performed to detect the expression of RUNX3 protein in tissues.All postoperative patients were followed up to investigate the effects of miR-130a and RUNX3 expression on the prognosis of patients with esophageal squamous cell carcinoma.【Results】Compared with adjacent tissues,the expression level of miR-130a in esophageal squamous cell carcinoma tissues was higher(P<0.05),while the expression level of RUNX3 protein was lower(P<0.05).The expression levels of miR-130a and RUNX3 protein in esophageal squamous cell carcinoma were negatively correlated(P<0.05).The protein expression levels of miR-130a and RUNX3 were obviously correlated with T stage,cancer differentiation and lymph node metastasis(P<0.05).Kaplan-Meier survival curve analysis showed that the median survival time of patients with esophageal squamous cell carcinoma of high miR-130a expression was obviously shorter than that of patients with low miR-130a expression(P<0.05).Also the result showed that the median survival time of patients with esophageal squamous cell carcinoma with low RUNX3 expression was obviously shorter than that of patients with high RUNX3 expression(P<0.05).Multivariate Cox analysis showed that T staging(T3-T4),lymph node metastasis,high expression of miR-130a,and low expression of RUNX3 were independent risk factors for poor prognosis in patients with esophageal squamous cell carcinoma(P<0.05).【Conclusion】The expression level of miR-130a is up-regulated and the expression level of RUNX3 protein is down-regulated in esophageal squamous cell carcinoma.The two are closely related to the poor prognosis of patients with esophageal squamous cell carcinoma.Patients with esophageal squamous cell carcinoma with high expression of miR-130a and low expression of RUNX3 may have a higher risk of poor prognosis.