GATA结合蛋白3在宫颈癌及癌前病变组织中的表达及其临床意义

Expression and Clinical Significance of GATA Binding Protein 3 in Cervical Cancer and Precancerous Lesions

【目的】探讨GATA结合蛋白3(GATA3)在宫颈癌及癌前病变组织中的表达及其临床意义。【方法】收集本院收治的162例宫颈手术标本,其中宫颈上皮内瘤变(CIN)Ⅰ35例、CINⅡ~Ⅲ43例、宫颈癌42例及正常宫颈42例,分别记为CINⅠ组、CINⅡ~Ⅲ组、宫颈癌组及对照组。对比四组对象临床资料及GATA3mRNA表达水平。对比宫颈癌组、癌前病变组(CINⅠ组+CINⅡ~Ⅲ组)患者的临床资料,Logistic多因素回归分析影响宫颈癌发生的危险因素,绘制受试者工作特征(ROC)曲线,以曲线下面积(AUC)评价GATA3mRNA水平对宫颈癌的诊断效能。【结果】四组GATA3mRNA组间比较,差异均有统计学意义(P<0.05);CINⅠ组、CINⅡ~Ⅲ组及宫颈癌组GATA3 mRNA水平均低于对照组(P<0.05);宫颈癌组GATA3mRNA均低于CINⅠ组和CINⅡ~Ⅲ组(P<0.05);CINⅡ~Ⅲ组GATA3mRNA低于CINⅠ组(P<0.05)。宫颈癌组生产次数、吸烟史占比、有宫颈癌家族史占比、性伴侣≥2个占比均高于癌前病变组(P<0.05),首次性生活年龄、首次生育年龄及GATA3mRNA则低于癌前病变组(P<0.05)。Logistic多因素分析结果显示,生产次数、性伴侣≥2个及GATA3mRNA均为影响宫颈癌发生的危险因素(P<0.05)。ROC曲线分析显示,宫颈组织中GATA3mRNA表达水平预测宫颈癌发生的最佳截断点为1.12,灵敏度、特异度及AUC分别为80.95%、83.33%、0.869。【结论】GATA3mRNA在宫颈癌、癌前病变组织中的表达均异常偏低,其对宫颈癌的诊断效能较高,可作为临床宫颈癌诊断的重要参考指标。

【Objective】To explore the expression and clinical significance of GATA binding protein 3(GATA3)in cervical cancer and precancerous lesions.【Methods】A total of 162 cases of cervical surgery specimens admitted to our hospital were collected,including 35 cases of cervical intraepithelial neoplasia(CIN)Ⅰ(the CINⅠgroup),43 cases of CINⅡ~Ⅲ(the CINⅡ~Ⅲgroup),42 cases of cervical cancer(the cervical cancer group)and 42 cases of normal cervix(the control group).The clinical data and GATA3 mRNA expression levels of the four groups were compared.The clinical data of patients with cervical cancer group and precancerous lesion group(CINⅠand CINⅡ~Ⅲ)were compared with the other groups.Logistic multivariate regression was analyzed the risk factors affecting cervical cancer.Receiver operating characteristic curve(ROC)and the area under the curve(AUC)were drawn to evaluate the diagnostic efficacy of GATA3 mRNA levels for cervical cancer.【Results】There were statistically significant differences in GATA3 mRNA among the four groups(P<0.05).GATA3 mRNA in the CINⅠ,CINⅡ~Ⅲ,and cervical cancer groups were all lower than those in the control group(P<0.05).GATA3 mRNA level in the cervical cancer group was lower in that in the CINⅠgroup and CINⅡ~Ⅲgroup(P<0.05).GATA3 mRNA level in the CINⅡ~Ⅲgroup was lower than that in the CINⅠgroup(P<0.05).The number of births,the percentage of smoking history,proportion of family history of cervical cancer,and the percentage of sexual partners≥2 in the cervical cancer group were higher than those in the precancerous lesion group(P<0.05),while the age of first sex,age of first childbearing and GATA3 mRNA were lower(P<0.05).Logistic multivariate analysis showed that the number of births,sex partners≥2 and GATA3 mRNA were all risk factors affecting cervical cancer(P<0.05).ROC analysis showed that the best cut-off point of GATA3 mRNA expression in cervical tissues for predicting the occurrence of cervical cancer was 1.12,and the sensitivity,specificity and AUC were 80.95%,83.33%,and 0.869,respectively.【Conclusion】The expression of GATA3 mRNA in cervical cancer and precancerous lesions is abnormally low,and its diagnostic efficiency for cervical cancer is relatively high.It can be used as an important reference index for clinical diagnosis of cervical cancer.