白芨三萜类化合物的提取工艺优化

Optimization of Extraction Technology of Bletilla striata Triterpenoids

以白芨根状茎为试验材料,采用超声波辅助乙醇提取法,通过单因素试验和正交试验优化白芨中三萜类化合物的提取工艺,探究乙醇体积分数、料液比、超声波处理时间和超声波处理温度对白芨三萜类化合物提取率的影响。结果表明:各因素对白芨三萜类化合物提取率的影响由大到小排列依次为料液比>乙醇体积分数>超声波处理时间>超声波处理温度,白芨三萜类化合物提取的最佳工艺条件为乙醇体积分数70%、料液比1∶40(g/mL)、超声波处理时间10 min、超声波处理温度40℃,经验证,在此提取工艺条件下,白芨三萜类化合物的平均提取率为0.645%。

In this study,the rhizome of Bletilla striata was used as the test material,and the single factor method and orthogonal experiment were used to optimize the ultrasonic-assisted ethanol extraction process of triterpenoids in Bletilla striata.The effects of ethanol volumn fraction,material-liquid ratio,ultrasonic time and ultrasonic temperature on the extraction rate of triterpenoids were studied.The results showed that the order of importance of influencing factors for the extraction rate of Bletilla striata triterpenoids was material-liquid ratio>ethanol volume fraction>ultrasonic time>ultrasonic temperature.The optimal extraction conditions for Bletilla striata triterpenoids were ethanol volume fraction 70%,material-liquid ratio 1∶40 (g/mL),ultrasonic time 10 min and ultrasonic temperature 40℃,under whichthe average extraction rate of Bletilla striata triterpenoids was 0.645%.