中药活血化瘀复方剂对SD大鼠的毒性研究

Study on toxicity of Chinese medicine compound agent for improving blood circulation and removing blood stasis on SD rats

目的 观察丹参饮、血府逐瘀汤、失笑散、活络效灵丹、桃红四物汤五首活血化瘀方对SD大鼠的毒性反应。方法 将72只SD大鼠随机分为丹参组、血府组、失笑组、桃红组、活络组、正常组,每组12只。丹参组给予丹参饮(含生药109.87 g·kg^(-1)·d^(-1));血府组给予血府逐瘀汤(含生药198.80 g·kg^(-1)·d^(-1));失笑组给予失笑散(含生药31.36 g·kg^(-1)·d^(-1));活络组给予活络效灵丹(含生药156.80 g·kg^(-1)·d^(-1));桃红组给予桃红四物汤(含生药148.96 g·kg^(-1)·d^(-1));正常组给予等体积的蒸馏水。6组大鼠每天灌胃给药1次,连续灌胃30 d后,停药恢复15 d。检测大鼠血液生化学和脏器湿重等指标。结果 与正常组比较,五首活血化瘀方组大鼠血液生化部分指标差异均有统计学意义(均P<0.05),如失笑组雄鼠和桃红组雄鼠的谷丙转氨酶均显著降低[(66.1±2.9),(74.9±10.3)vs(93.7±16.2)U·L^(-1)],失笑组雄鼠的白蛋白[(28.8±0.3)vs(31.2±0.8)g·L^(-1)]和总蛋白[(48.2±0.9)vs(52.9±0.7)g·L^(-1)]均显著降低,停药15 d后恢复正常;部分脏器湿重差异均有统计学意义(均P<0.05),如失笑组雄鼠的肝[(9.7±0.4)vs(10.6±0.5)g]和胸腺[(0.6±0.1)vs(0.8±0.1)g]湿重均显著降低;活络组雌鼠和桃红组雌鼠的胃湿重均显著增高[(2.5±0.4),(2.5±0.1)vs(1.5±0.3)g]。大鼠的体表、脏器、一般状态等未见明显异常。结论 五首活血化瘀方临床拟用剂量安全。

Objective To observe the toxic effects of five herbal compounds for improving blood circulation and removing blood stasis,including Danshen Yin,Xuefu Zhuyu Tang,Shixiao San,Huoluoxiaoling Dan,and Taohong Siwu Tang on SD rats.Methods Seventy-two SD rats were randomly divided into Danshen,Xuefu,Shixiao,Taohong,Huoluo and normal groups with 12 rats per group.The five treatment groups were respectively given Danshen Yin(containing crude drug 109.87 g·kg^(-1)·d^(-1)),Xuefu Zhuyu Tang(containing crude drug 198.80 g·kg^(-1)·d^(-1)),Shixiao San(containing crude drug 31.36 g·kg^(-1)·d^(-1)),Huoluoxiaoling Dan(including crude drug 156.80 g·kg^(-1)·d^(-1)) and Taohong Siwu Tang(containing crude drug 148.96 g·kg^(-1)·d^(-1)) by intragastric administration for 30 days.The normal group was given an equal volume of distilled water by gavage,and then the drug was stopped and recovered for 15 days.Indicators such as blood biochemistry and wet weight of organs were detected in rats.Results Compared with the normal group,the blood biochemical indexes in the five herbal compounds group had statistically significant differences( all P < 0.05),such as the alanine aminotransferase of male rats in the Shixiao group and the Taohong group was significantly reduced [( 66.1 ± 2.9),( 74.9 ± 10.3) vs( 93.7 ± 16.2) U · L^(-1)],the albumin of male rats in the Shixiao group [( 28.8 ± 0.3) vs( 31.2 ± 0.8) g·L^(-1)]and total protein[( 48.2 ± 0.9) vs( 52.9 ± 0.7) g·L^(-1)]were significantly reduced,15 days after stopping the drug return to normal;the differences in wet weight of organs were statistically significant( all P < 0.05),such as the liver of male rats in the Shixiao group [( 9.7 ± 0.4) vs( 10.6 ± 0.5) g] and thymus wet weight [( 0.6 ± 0.1) vs( 0.8 ± 0.1) g]decreased significantly;the wet stomach weight of female rats in Huoluo group and Taohong group increased significantly [( 2.5 ± 0.4),( 2.5 ± 0.1) vs( 1.5 ± 0.3) g].There were no obvious abnormalities in the body surface,organs,and general state of the rats.Conclusion The clinical dosage of five herbal compounds for improving blood circulation and removing blood stasis is safe.