低温微创冷冻减脂动物模型的构建

Experimental study on the construction of animal model of low-temperature minimally invasive cryolipolysis

目的:探讨建立大鼠皮下脂肪微创冷冻减脂实验的动物模型。方法:将大鼠随机分为低温组(0℃组、-20℃组、-40℃组、-60℃组、-80℃组)以及室温对照组(Control组),利用不同低温干预造成大鼠皮下脂肪局部冷损伤,观察脂肪厚度及病理学改变。结果:超声示各低温组均有不同程度脂肪厚度减少,在无皮肤损伤情况下低温-60℃干预60 s时脂肪厚度减少最为明显(P<0.01)。病理学显示局部冷冻后随着温度的降低,炎症细胞浸润与脂肪细胞破坏愈加明显,在低温-60℃干预60 s 3 d后脂肪组织炎症细胞浸润达到峰值(P<0.01)。与Control组比,-60℃组脂肪组织内NF-κB-p65在第2周时表达明显上升,TGF-β1在第4周时阳性表达明显上升(P<0.01)。-20℃组TUNEL染色细胞凋亡逐渐增多,-60℃组干预60 s3 d后细胞凋亡最明显(P<0.01)。结论:采用低温探针技术在低温-60℃干预60 s条件下,可建立稳定的SD大鼠皮下脂肪微创冷冻减脂模型。

Objective: To establish a minimally invasive Cryolipolysis model of subcutaneous fat in rats.Methods: The rats were randomly divided into low temperature group(0 ℃ group,-20 ℃ group,-40 ℃group,-60 ℃ group,-80 ℃ group) and room temperature Control group(Control group). Local cold injury of subcutaneous fat in rats was caused by different low temperature intervention, and fat thickness and pathological changes were observed. Results: B-ultrasonography showed that fat thickness decreased to varying degrees in all low-temperature test groups, with the most obvious reduction in fat thickness. No epidermal damage was found when the temperature was-60 ℃ and the contact time was 60 s, which was in line with the requirements of the Cryolipolysis research model(P<0.01). Pathology showed that with the decrease of temperature after local freezing,the inflammatory reaction in adipose tissue was gradually enhanced, and the infiltration of inflammatory cells and destruction of adipose cells became more obvious(P<0.01). The infiltration of inflammatory cells in adipose tissue reached the peak when the temperature was-60 ℃ and the contact time was 60 s(P<0.01). Immunohistochemistry showed that NF-κB-p65 expression was obvious at week 2 and TGF-β1 expression was obvious at week 4, which were significantly different from control group(P<0.01). When the temperature was as below-20 ℃, the apoptosis of Tunel stained adipocytes increased gradually, and the apoptosis was most obvious when the low temperature(-60 ℃) was intervened 60 s. Conclusion: Under the condition of 60 seconds’ intervention at-60 ℃, a stable minimally invasive Cryolipolysis model of subcutaneous fat in SD rats can be established by using low-temperature probe technology.