杨梅全基因组的GATA转录因子鉴定、功能及进化分析

Genome-wide identification, functional and evolutionary analysis of GATA transcription factor family in Myrica rubra

对杨梅GATA转录因子进行全基因组鉴定,并预测其理化特性、染色体定位、基因结构、蛋白保守序列等基本特征.结果表明,26个GATA转录因子随机分布在8条染色体上,共分为4个亚家族,并且各亚家族的成员在保守基序、结构域及基因结构上均存在一定的差异性.物种共线性结果表明,基因复制和片段性的重复事件可能是部分MrGATA演变的主要驱动力,MrGATA在不同组织和发育时期的表达数据说明各亚家族之间也存在功能上的差异.根据顺式作用元件及表达模式推测了GATA转录因子家族成员的功能.这些数据为探究杨梅MrGATA功能以及分子育种提供了依据.

The genome-wide identification of GATA transcription factors in Myrica rubra was performed, and the physical and chemical characteristics, chromosome location, gene structure, duplication pattern, conserved domain, and other basic parameters were predicted. The results showed that 26 GATA transcription factors were randomly distributed on 8 chromosomes;they were divided into 4 subfamilies, with the members of each subfamily differing in conserved motif, domain and gene structure to some extents. Species collinear analysis indicated that gene replication and fragmented repeated events might be the main driving forces for the evolution of some MrGATA genes. The functional differences among subfamilies were further verified by the expression patterns of MrGATA in different tissues and at various developmental stages. Moreover, the functions of members of the MrGATA transcription factor family were speculated based on cis-acting elements and their expression patterns. The study provided a theoretical basis for exploring the function of GATA gene in M.rubra as well as the molecular breeding of M.rubra.