川楝子亚急性毒性实验研究

Experimental Study on Subacute Toxicity of MeLia Toosendan

目的采用亚急性毒性实验明确川楝子的中毒剂量及对肝、肾组织的损伤程度,阐明川楝子的肝、肾毒性作用,为临床安全合理应用提供工作基础。方法以川楝子为示例药物,以大鼠为研究对象,川楝子组灌胃相应药物溶液,连续给予21 d,末次给药后取血,分离血清,采用全自动生化分析仪检测肝、肾功能ALT、AST、CREP、UREA、Tg,CHO、TP指标。结果川楝子可显著提高血清AST、ALT含量,有统计学意义(P<0.05);CHO、TP含量显著下降(P<0.05);Tg,CREP含量差异显著(P<0.05)。结论川楝子具有一定的肝毒性,并呈现剂量依赖性,2.5714 g/kg剂量时有明显的肝毒性,故临床应用剂量不可超过2.5714 g/kg。

Objective:To determine the toxic dose of MeLia toosendan and the degree of damage to liver and kidney tissues by subacute toxicity experiment,to clarify the liver and kidney toxicity of MeLia toosendan,and to provide a working basis for safe and rational clinical application.Methods:MeLia toosendan was taken as an example drug,and rats as the research object.MeLia toosendan group was given the corresponding drug solution by intragastric administration for 21 days,and the blood was collected after the last administration,serum was separated.The liver and kidney functions were detected by automatic biochemical analyzer to detect the ALT,AST,CREP,UREA,Tg,CHO,TP indicators of liver and kidney function.Results:MeLia toosendan could significantly increase serum AST and ALT contents,with statistical significance(P<0.05)and significantly decreased CHO and TP contents(P<0.05)and Tg and CREP contents were significantly different(P<0.05).Conclusion:MeLia toosendan has a certain degree of hepatotoxicity,and is dose-dependent.The dose of 2.5714 g/kg has obvious hepatotoxicity,so the clinical application dose should not exceed 2.5714 g/kg.