响应面试验优化超声法提取菊芋花青素工艺

Optimization of Ultrasonic Extraction of Anthocyanins from Helianthus tuberosus L.by Response Surface Methodology

为确定从菊芋块茎表皮提取花青素的最佳提取工艺,依托单因素试验,采用Plackett-Burman等试验研究料液比和超声时间等因素对菊芋块茎表皮花青素获得值的影响,采用响应面和遗传算法对提取菊芋块茎表皮花青素的工艺进行优化,利用遗传算法验证工艺最优情况。最优条件为料液比1∶15(g/mL),超声时间113 min,超声温度40℃,乙醇体积分数70%,盐酸浓度0.3 mol/L。在此条件下,花青素的理论获得值为35 mg/g,与试验值34.52 mg/g基本相符。该优化工艺参数可为菊芋块茎表皮花青素的提取提供一定的参考依据。

In order to determine the best extraction process of anthocyanins from Helianthus tuberosus L.tuber epidermis,based on single factor experiment,Plackett-Burman and other experiments are used to study the effects of factors such as solid-liquid ratio and ultrasonic time on the earned value of anthocyanins from Helianthus tuberosus L.tuber epidermis.Response surface methodology and genetic algorithm are used to optimize the extraction process of anthocyanins from Helianthus tuberosus L.tuber epidermis,and genetic algorithm is used to verify the optimal process.The optimal conditions are as follows:solid-liquid ratio is 1∶15(g/mL),ultrasonic time is 113 min,ultrasonic temperature is 40℃,ethanol volume fraction is 70%,hydrochloric acid concentration is 0.3 mol/L.Under such conditions,the theoretical earned value of anthocyanin is 35 mg/g,which is basically consistent with the experimental value of 34.52 mg/g.The optimized process parameters can provide certain reference data for the extraction of anthocyanins from the tuber epidermis of Helianthus tuberosus L..