CDH1、FANCB和APC基因多态性与中国人群肺癌易感性的关系

Association of CDH1,FANCB and APC Gene Polymorphisms with Lung Cancer Susceptibility in Chinese Population

背景与目的肺癌是全球癌症相关死亡的主要原因,单核苷酸多态性(single nucleotide polymorphism,SNP)是导致肺癌发生的重要因素之一,但其机制仍未阐明。本研究拟探讨CDH1、FANCB、APC基因SNP与肺癌遗传易感性的关系。方法采用病例对照研究方法,收集来自天津医科大学总医院肺部肿瘤外科270例肺癌病例,同时收集445名健康志愿者的血液样本作为对照,并提取基因组DNA,使用Taqman~?SNP基因分型试剂盒进行基因分型,分析CDH1基因rs201141645、FANCB基因rs754552650和APC基因rs149353082三个SNPs位点在中国人群中的分布。采用卡方检验和Logistic回归分析探索不同基因型与肺癌发病风险之间的关系。结果FANCB基因rs754552650位点的AA、A/G和GG基因型的分布频率在对照组中分别为27.2%、52.6%和20.2%。AA和A/G基因型分布频率在病例组中分别为93.7%、6.3%,未检测到GG基因型。FANCB基因的rs754552650位点的A/G基因型在病例组和对照组中存在显著差异。携带者患肺癌的风险明显降低(OR=0.035,95%CI:0.020-0.062,P<0.001)。CDH1基因rs201141645 A/C和CC基因型仅存在于对照组中。此外,在病例组中仅发现1个样本存在APC基因rs149353082 C/G基因型。结论在中国人群中,FANCB基因rs754552650 A/G基因型携带者患肺癌的风险明显降低。

Background and objective Lung cancer is the main cause of cancer-related death globally.Single nucleotide polymorphism(SNP)is one of the important factors leading to the occurrence of lung cancer,but its mechanism has not been elucidated.This study intends to investigate the relationship between SNPs of CDH1,FANCB,APC genes and lung cancer genetic susceptibility.Methods The case-control study design was used.We collected blood samples from 270 lung cancer cases in the Department of Lung Cancer Surgery,Tianjin Medical University General Hospital,as well as blood samples from 445 healthy volunteers as controls,and extracted genomic DNA for genotyping using the Taqman?SNP genotyping kit.The distribution of three SNP loci of CDH1 gene rs201141645,FANCB gene rs754552650 and APC gene rs149353082 in Chinese population was analyzed.Chi-square test and Logistic regression were used to analyze the relationship between different genotypes and the risk of lung cancer.Results The distribution frequencies of AA,A/G and GG genotypes at rs754552650 of FANCB gene in the control group were 27.2%,52.6%and 20.2%,respectively.The distribution frequencies of AA and A/G genotypes were 93.7%and 6.3%in the case group,respectively,and no GG genotype was detected.The A/G genotype of the rs754552650 locus of the FANCB gene was significantly different between the case group and the control group.Compared with the carriers of AA genotype,the individuals with FANCB rs754552650 A/G genotype had a lower risk of lung cancer(OR=0.035,95%CI:0.020-0.062,P<0.001).CDH1 gene rs201141645 A/C and CC genotypes only existed in the control group.In addition,only 1 sample was found to have APC rs149353082 genotype in the case group.Conclusion In the Chinese population,the lung cancer risk of the individuals with FANCB rs754552650 A/G genotype was significantly decreased.