盐胁迫下六妹羊肚菌菌丝体的理化性状

Physicochemical properties of Morchella sextelata mycelium under salt stress

以六妹羊肚菌M3号为研究对象,考察盐胁迫对其菌丝体的影响。采用水提醇沉法、超声波辅助提取法、DPPH还原法、H _(2)O_(2)/Fe体系法和铁氰化钾还原法,测定盐胁迫不同时间下菌丝显微结构、胞内外多糖含量、DPPH自由基、羟自由基清除率及还原能力的情况变化。结果显示,随盐胁迫时间的增加,羊肚菌菌丝直径缓慢减小;含盐菌丝多糖含量在3 h时高于正常菌丝多糖含量,为12.74 mg/g,之后含量一直低于正常菌丝;含盐胞外多糖含量低于正常胞外多糖含量,但都呈先上升后下降趋势,在12 h含量最高,分别为0.14、0.29 mg/mL;盐胁迫促进羊肚菌胞内外多糖对DPPH自由基和羟自由基的清除率的提高,尤其是对DPPH自由基的清除能力,胞内多糖清除能力最高提升了3.47倍,胞外多糖最高提升了6.72倍;还原力上胁迫对胞内多糖的影响大于胞外多糖。综上所述,该研究为羊肚菌盐胁迫下生理活性的探索提供一定理论基础。

Taking Morchella sextelata M3 as the research object,the effect of salt stress on mycelium was investigated.Water extraction and alcohol precipitation method,ultrasonic assisted extraction method,DPPH reduction method,H_(2)O_(2)/Fe system method and potassium ferricyanide reduction method were used to determine the changes of mycelium microstructure,intracellular and extracellular polysaccharide content,DPPH free radical,hydroxyl radical scavenging rate and reduction ability under salt stress at different times.The results showed that the mycelium diameter decreased slowly with the increase of salt stress time.The polysaccharide content of salt-containing mycelia was higher than that of normal mycelia at 3 h,which was 12.74 mg/g,and then the content was lower than that of normal mycelia.The content of extracellular polysaccharides under salt stress was lower than that of normal content,but they both increased first and then decreased.The highest content was 0.14 mg/mL and 0.29 mg/mL at 12 h,respectively.Salt stress promoted the scavenging rate of DPPH free radical and hydroxyl free radical,especially DPPH free radical scavenging capacity,intracellular polysaccharide clearance capacity increased by 3.47 times,and extracellular polysaccharide increased by 6.72 times.The effect of reducing power on intracellular polysaccharide was greater than that on extracellular polysaccharide.In summary,this study provides a theoretical basis for exploring the physiological activity of Morchella sextelata under salt stress.