硒代4-叔丁基杯[4]芳烃的合成及体外抗氧化活性研究

Synthesis and Antioxidant Activity Evaluation of Selenium 4-tert-Butylcalix[4]arene

通过对于4-叔丁基杯[4]芳烃结构中羟基基团的硒化修饰反应,合成了一种新的具有GPX活性的模拟物-硒代4-叔丁基杯[4]芳烃,并用质谱和核磁共振光谱对该GPX模拟物的结构进行了确证。其GPX活力可达137.22U/μmol,进一步建立了由Fe^(2+)/Vc诱导的牛心线粒体损伤体系来研究该GPX模拟物的体外抗氧化活性,结果显示该模拟物表现出良好的体外抑制线粒体氧化损伤的生物活性。

By a selenization modification reaction for the hydroxyl group in the 4-tert-Butylcalix[4]arene,a new GPX mimic selenium 4-tert-Bu-tylcalix[4]arene was synthesized,its structure was confirmed by 1H NMR and ESI-MS.Its GPX activity reached 137.22 U/μmol,we established a Fe^(2+)/Vc-induced mitochondrial damage system to evaluate the biological activity of the GPX mimic in vitro.The results showed that the GPX mimic perform effective biological activity to anti-oxidative damage of the mitochondria in vitro.