扫描透射电子显微镜技术及其在中药研究中的应用

Scanning Transmission Electron Microscopy and Its Application in Traditional Chinese Medicine Research

扫描透射电子显微镜(STEM)兼具透射电子显微镜(TEM)和扫描电子显微镜(SEM)的优势,具有高分辨成像、强原子衬度敏感以及图像易懂、直观等优点,已成为材料微结构和微区分析的必要性工具.中药研究有着悠久历史,显微技术一直是研究药材微特征和作用机制的重要手段之一,具有其他手段不可替代的作用.综述了STEM的原理、方法、技术特点,以及其在中药显微结构和纳米颗粒等方面的应用,以期为进一步扩展中药微特征领域的研究作参考.

Scanning transmission electron microscopy(STEM), a necessary tool for the microstructure of materials and microarea analysis, combines the advantages of transmission electron microscopy(TEM) and scanning electron microscopy(SEM), and has the advantages of high-resolution imaging, strong atomic contrast sensitivity, and easy-tounderstand visual images. Traditional Chinese medicine research has a long history. Microscopic technology has always been one of the important means to study the micro-characteristics and mechanism of medicinal materials, and is irreplaceable to the other technologies. The principles, methods and technical characteristics of STEM, as well as its application in the microstructure and nanoparticles of traditional Chinese medicine has been reviewed, in order to provide reference for the further expansion of the research on the micro-characteristics of traditional Chinese medicine.