CRISPR/Cas9基因编辑技术在丝状真菌中的应用

Research Progress on Application of CRISPR/Cas9 Gene Editing Technique in Filamentous Fungi

随着对丝状真菌基因水平研究的不断深入,CRISPR/Cas9技术作为先进的基因编辑技术,已被广泛应用于丝状真菌的基因编辑。探究了CRISPR/Cas9系统在不同丝状真菌中的应用情况,主要从sgRNA的构建与表达、Cas9蛋白的改造与表达、不同的DNA双链断裂修复(DNAdouble-strandbreak,DSB)方式等方面进行概述,并对编辑效率、脱靶效应进行总结,旨在为今后丝状真菌中CRISPR/Cas9系统的构建及改良提供思路。

With the development of the research on filamentous fungi gene level,CRISPR/Cas9 technology is an advanced gene editing technology,which has been widely used in filamentous fungi gene editing.This article explored the application of the CRISPR/Cas9 system in different filamentous fungi.It included an overview of the construction and expression of sgRNA,the transformation and expression of Cas9 protein,and different DNA double-strand break(DSB)methods,and the editing efficien⁃cy and off-target effects were also summarized.It was expected to provided ideas for the construction and improvement of the CRISPR/Cas9 system in the future.