基于镧系金属有机框架纳米酶构建电化学生物传感器用于多巴胺的灵敏检测

Lanthanide metal-organic frameworks based nanozyme electrochemical sensor with high sensitivity for depamine detection

将三种镧系元素Ce、Tb、Gd为中心原子在电化学还原的石墨烯上原位合成了的金属有机框架(Metal-Organic Frameworks,MOFs)作为纳米酶,在玻碳电极表面构建了电化学生物传感器,并用于多巴胺(DA)的灵敏检测。应用X射线衍射仪(XRD)、X射线光电子能谱仪(XPS)等方法对所制备的MOFs进行了表征,并用循环伏安法(CV)和差分脉冲伏安法(DPV)研究了不同M-MOF(M=Ce、Tb、Gd)纳米酶的性能。根据研究结果,基于Ce-MOF构建的电化学传感器对多巴胺(DA)的反应具有最高的灵敏度和选择性,在0.1~100μM的范围内,其灵敏度为0.611 mA mM^(-1),检出限为6.47×10^(-8) M(S/N=3)。同时,该传感器还展现了良好的稳定性能和抗干扰能力,并且成功应用在实际样品测定中。

Three Lanthanides(Ce,Tb,Gd),as the center atoms,were used to in situ synthesize metal-organic Frameworks(MOFs)on electrochemically reduced graphene.The electrochemical biosensors were constructed on the surface of glassy carbon electrode and used for sensitive detection of dopamine(DA).The morphology and structure of M-MOF(M=Ce、Tb、Gd)were characterized by X-ray diffraction(XRD)and X-ray photoelectron spectroscopy(XPS),and the properties of different M-MOF(M=Ce,Tb,Gd)nanozymes were studied by cyclic voltammetry(CV)and differential pulse voltammetry(DPV).According to the results,Ce-MOF dropped metal-organic frameworks based nanozyme electrochemical sensor exhibited a high sensitivity and selectivity for dopamine(DA),with the sensitivity of 0.611 mA·mM^(-1 )in the range of 0.1~100μM and a lower detection limit of 6.47×10^(-8) M(S/N=3).In addition,the sensor showed satisfactory stability and has been successfully applied to the detection of DA in samples.