Clara细胞10 kD蛋白通过调控T细胞反应治疗小鼠变应性鼻炎研究

Study on Clara cell 10 kD protein regulating T cell response in a mouse model of allergic rhinitis

目的:探讨Clara细胞10 kD蛋白(CC10)对变应性鼻炎(AR)小鼠模型T细胞反应的作用。方法:卵清蛋白(OVA)免疫小鼠,建立AR模型,探讨CC10对AR的影响。最后1次鼻腔激发后评估小鼠鼻腔症状。收集鼻组织和鼻腔灌洗液(NLF),测定AR小鼠嗜酸性粒细胞、杯状细胞数及IL-5、IL-17、IL-10、TGF-β1、FOXP3水平。结果:激发期间给予CC10可显著减少AR小鼠搔鼻次数、嗜酸性粒细胞和杯状细胞数,降低IL-5、IL-17水平(P<0.05),但可增加IL-10、TGF-β1和FOXP3表达(P<0.05)。结论:CC10可通过调控T细胞反应减轻AR小鼠鼻腔炎症。

Objective:To investigate effects of Clara cell 10 kD protein(CC10)on T cells response of a mouse model of allergic rhinitis(AR).Methods:Mice were immunized and challenged with ovalbumin(OVA)to establish an AR model to study effects of CC10 on AR. Intranasal administration of CC10 was then performed. Nasal symptoms were estimated after last nasal challenge. Nasal tissue and nasal lavage liquid(NLF)were collected. Number of eosinophils and goblet cells and levels of IL-5,IL-17,IL-10,TGF-β1 and FOXP3 in AR mice were measured.Results:Administration of CC10 during challenge significantly reduced mouse nasal rubs,number of eosinophils and goblet cells,decreased levels of IL-5,IL-17(P<0.05),but increased levels of IL-10,TGF-β1 and FOXP3 expressions in AR mice(P<0.05).Conclusion:CC10 can regulate T cell responses to alleviate nasal allergic inflammation in AR mice.