超高效液相色谱法测定罗汉果中7种成分含量

Simultaneous Determination of Seven Components in Siraitiae Fructus by Ultra Performance Liquid Chromatography

目的建立超高效液相色谱法(UPLC)测定罗汉果中11-O-罗汉果苷Ⅴ、罗汉果皂苷Ⅴ、赛门苷Ⅰ、罗汉果苷Ⅳ、罗汉果苷Ⅲ、罗汉果苷Ⅲe、罗汉果苷Ⅱe的含量。方法采用Waters BEH C_(18)色谱柱(50 mm×2.1 mm,1.7μm),流动相为水-乙腈溶液,梯度洗脱;柱温35℃;流速为0.3 mL·min^(-1);进样量为1μL;采用紫外检测器进行定量测定,检测波长203 nm;采用质谱检测器进行定性鉴别,检测模式为选择离子监测,监测离子的质荷比为1307.8,1309.8,1147.8,1147.8,985.7,985.7和823.7。结果11-O-罗汉果苷Ⅴ、罗汉果皂苷Ⅴ、赛门苷Ⅰ、罗汉果苷Ⅳ、罗汉果苷Ⅲ、罗汉果苷Ⅲe、罗汉果苷Ⅱe分别在0.50~20.10,5.03~201.20,0.51~20.24,0.52~20.63,0.51~20.22,0.51~20.56,0.50~19.92μg·mL^(-1)范围内线性关系良好(r≥0.999);平均回收率分别为100.7%,98.4%,100.4%,99.0%,100.4%,99.2%和99.2%,RSD分别为1.9%,1.0%,2.0%,1.9%,1.3%,0.8%和2.1%;质谱鉴别结果与紫外检出情况一致。结论该方法操作便捷、结果准确,重复性好,可用于同时测定罗汉果中的11-O-罗汉果苷Ⅴ、罗汉果皂苷Ⅴ、赛门苷Ⅰ、罗汉果苷Ⅳ、罗汉果苷Ⅲ、罗汉果苷Ⅲe、罗汉果苷Ⅱe的含量。

Objective To establish an ultra-performance liquid chromatography method(UPLC)for determination of 11-Oxomogroside Ⅴ,Mogroside Ⅴ,Siamenoside Ⅰ,Mogroside Ⅳ,Mogroside Ⅲ,Mogroside Ⅲe and Mogroside Ⅱe in Siraitiae Fructus.Methods The analysis was performed on a Waters BEH-C_(18) column(50 mm×2.1 mm,1.7μm),with the mobile phase of acetonitrile-aqueous solution in gradient mode.The column temperature was 35℃.The flow rate was 0.3 mL·min^(-1).The injection volume was 1μL.An ultraviolet detector setting at 203 nm was used for quantitative analysis.A mass spectrum detector in the selected ion monitoring model was used for qualitative analysis by monitoring ions of 1307.8,1309.8,1147.8,1147.8,985.7,985.7,and 823.7,respectively.Results 11-Oxomogroside Ⅴ,Mogroside Ⅴ,Siamenoside Ⅰ,Mogroside Ⅳ,Mogroside Ⅲ,Mogroside Ⅲe and Mogroside Ⅱe showed good linear relationships within the ranges of 0.50-20.10,5.03-201.20,0.51-20.24,0.52-20.63,0.51-20.22,0.51-20.56,0.50-19.92μg·mL^(-1)(r≥0.999),respectively.The average recoveries were 100.7%,98.4%,100.4%,99.0%,100.4%,99.2% and 99.2%,with RSD of 1.9%,1.0%,2.0%,1.9%,1.3%,0.8% and 2.1%,respectively.Qualitative results matched well with quantitative results.Conclusion The UPLC method is simple,accurate,and specific,which can be used to determine 11-Oxomogroside Ⅴ,Mogroside Ⅴ,Siamenoside Ⅰ,Mogroside Ⅳ,Mogroside Ⅲ,Mogroside Ⅲe and Mogroside Ⅱe in Siraitiae Fructus.