CYP1A1、TSLPR、CCR3在小儿特应性皮炎中的表达及与临床特征相关性研究

Expression of CYP1A1,TSLPR and CCR3in atopic dermatitis in children and its correlation with clinical characteristics

目的 探究细胞色素P4501A(cytochrome P4501A,CYP1A1)、胸腺基质淋巴细胞生成素受体(thymic stromal lymphopoietin receptors, TSLPR)、CC趋化因子受体3(CC chemokine receptor 3,CCR3)在小儿特应性皮炎中的表达情况,并分析其表达与临床特征的相关性。方法 选取我院收治的特应性皮炎患儿89例作为特应性皮炎组,同期选取行健康体检的健康儿童80例作为健康组。采集两组入组后第2天空腹静脉血3 mL,在密度梯度离心处理下获得外周血单个核细胞,采用实时荧光定量聚合酶链反应测定CYP1A1、TSLPR表达量,采用荧光抗体标记、流式细胞仪测定外周血单个核细胞中CCR3表达水平。分析CYP1A1、TSLPR、CCR3在小儿特应性皮炎中的表达及与临床特征相关性。结果 与健康组比较,特应性皮炎组CYP1A1、TSLPR、CCR3表达均升高(P<0.05)。CYP1A1、TSLPR、CCR3表达与特应性皮炎患儿性别、年龄、伴随症状无关(P>0.05);CYP1A1、TSLPR、CCR3表达与特应性皮炎患儿疾病严重程度、家族过敏史、特应性家族史、个人过敏史相关,与轻度、中度、无家族过敏史、无特应性家族史、无个人过敏史的患儿相比,重度、有家族过敏史、有特应性家族史、有个人过敏史的患儿CYP1A1、TSLPR、CCR3表达升高(P<0.05)。Pearson相关性分析显示,CYP1A1、TSLPR、CCR3之间均呈正相关(P<0.05)。ROC曲线分析CYP1A1、TSLPR、CCR3对小儿特应性皮炎的预测价值结果显示,与CYP1A1、TSLPR、CCR3单项预测相比,三项联合的预测价值较高(P<0.05)。结论 CYP1A1、TSLPR、CCR3在小儿特应性皮炎中表达升高,且与患儿疾病严重程度、家族过敏史、特应性家族史、个人过敏史相关,临床上可采用三者联合预测此病。

Objective To investigate the expression of cytochrome P4501A(CYP1A1), thymic stromal lymphopoietin receptor(TSLPR) and CC chemokine receptor 3(CCR3) in children with atopic dermatitis and to analyze their correlation with clinical characteristics. Methods A total of 89 children with atopic dermatitis treated in our hospital were selected as atopic dermatitis group, and 80 healthy children who underwent physical examination in our hospital during the same period were selected as healthy group. Three mL of fasting venous blood was collected from the two groups on the 2nd day after enrollment, and peripheral blood mononuclear cells were obtained under density gradient centrifugation. The expressions of CYP1A1 and TSLPR were detected by real-time quantitative PCR(RT-qPCR), and CCR3 expression in peripheral blood mononuclear cells was measured by fluorescent antibody labeling and flow cytometry. The expressions of CYP1A1, TSLPR and CCR3 in children with atopic dermatitis and their correlation with clinical characteristics were analyzed. Results Compared with healthy group, the expressions of CYP1A1, TSLPR and CCR3 in atopic dermatitis group increased(P<0.05). The expressions of CYP1A1, TSLPR and CCR3 were not correlated with age, gender and accompanying symptoms of atopic dermatitis, suggesting no significant difference(P>0.05). The expressions of CYP1A1, TSLPR and CCR3 were correlated with disease severity, family allergy history, family history of atopic dermatitis, and personal allergic history. Compared with the patients with mild and moderate atopic dermatitis, no family history of atopic dermatitis, no family history of atopic dermatitis and no personal allergy, the expression of CYP1A1, TSLPR and CCR3 in patients with severe atopic dermatitis, family history of allergy, family history of atopic dermatitis, and personal history of allergy were significantly higher(P<0.05). Pearson correlation analysis showed positive correlation between CYP1A1, TSLPR and CCR3(P<0.05). ROC curve analysis of the predictive value of CYP1A1, TSLPR and CCR3 in children with atopic dermatitis showed that combined detection of CYP1A1, TSLPR and CCR3 in predicting atopic dermatitis in children was higher than that of CYP1A1, TSLPR and CCR3 alone(P<0.05). Conclusion The expressions of CYP1A1, TSLPR and CCR3 in children with atopic dermatitis increase, which is related to the severity of the disease, family history of allergy, family history of atopic dermatitis, and personal history of allergy. Therefore, combined detection of the three indicators can be used to predict atopic dermatitis in clinical practice.