猪源大肠杆菌的分离鉴定及耐药性分析

Isolation,Identification and Drug Resistance Analysis of E.coli From Pigs

旨在了解和掌握新乡市某规模化猪场大肠杆菌的耐药情况及耐药基因类型,为猪细菌病及其耐药性研究奠定基础。本研究从该猪场采集样本,通过平板划线法进行大肠杆菌的分离纯化,采用K-B纸片法进行药敏试验,并对多种耐药性基因进行PCR检测。试验共分离鉴定出15株大肠杆菌。药敏试验结果表明,分离菌株对氨苄西林、万古霉素、复方新诺明、氯霉素、四环素、庆大霉素、左氧氟沙星等多种药物具有多重耐药性;PCR扩增结果显示,这些菌株携带有氯霉素类的floR基因(100%)、β-内酰胺类的blaTEM基因(80.0%)、四环素tetM的基因(26.7%)等耐药基因,可能是导致大肠杆菌对相应抗生素产生耐药的原因。综上所述,这些猪源分离菌株大多为多重耐药大肠杆菌。

The purpose of this study was to understand and grasp the drug resistance status and drug resistance gene types of E.coli in a large-scale pig farm in Xinxiang City,so as to lay a foundation for the study of swine bacterial disease and its drug resistance.In this study,samples were collected from the pig farm,and E.coli was isolated and purified by plate marking method,K-B disk method was used for drug sensitivity test,and PCR detection of multiple drug resistance genes was conducted.A total of 15 strains of E.coli were isolated and identified.Drug sensitivity test showed that these isolated strains were multidrug-resistant to ampicillin,vancomycin,compound sulfamethoxazole,chloramphenicol,tetracycline,gentamicin,levofloxacin and other drugs.The results of PCR amplification showed that these isolated strains carried chloramphenicol floR gene(100%),β-lactam blatem gene(80.0%)and tetracycline tetM gene(26.7%),which may be responsible for the resistance of E.coli to the corresponding genes.To sum up,most of these procine isolates are multidrug-resistant E.coli.