慢性心力衰竭患者血清MCP-3,NRG-1β和CF6水平表达与疾病严重程度的相关性研究

Study on the Correlation between the Expression of Serum MCP-3,NRG-1β and CF6 Levels in Patients with Chronic Heart Failure and the Severity of the Disease

目的 分析慢性心力衰竭(chronic heart failure,CHF)患者血清单核细胞趋化蛋白-3(monocyte chemoattractant protein-3,MCP-3),神经调节蛋白1β(neuregulin 1β,NRG-1β)和偶联因子6(coupling factor 6,CF6)水平表达及与疾病严重程度的相关性。方法 选取2019年8月~2020年8月张家口市第一医院收治的慢性心力衰竭患者120例作为慢性心力衰竭组,另外选取70例健康人作为健康组,采用酶联免疫吸附(enzyme-linked immunoassay,ELISA)法检测MCP-3,NRG-1β和CF6水平,分析MCP-3,NRG-1β和CF6在健康组与慢性心力衰竭组的表达及与疾病严重程度的关系。结果 慢性心力衰竭组MCP-3(0.52±0.05 ng/L),NRG-1β(6.01±0.62 ng/ml)和CF6(304.64±30.58pg/ml)表达相对于健康组升高(0.10±0.01 ng/L,3.25±0.32 ng/ml,201.62±21.36 pg/ml),差异均具有统计学意义(t=69.400,34.630,24.860,均P<0.05)。MCP-3,NRG-1β和CF6随着慢性心力衰竭严重程度的加重升高加剧,差异具有统计学意义(F=15.117~41.816,均P<0.05)。ROC曲线显示,与MCP-3(AUC=0.842,95%CI=0.701~0.984),NRG-1β(AUC=0.851,95%CI=0.718~0.984)和CF6(AUC=0.873,95%CI=0.746~1.000)单项诊断相比,三项联合检测对慢性心力衰竭的诊断价值较高(AUC=0.899,95%CI=0.766~1.030),差异有统计学意义(均P<0.05)。相关性分析显示MCP-3与NRG-1β和CF6均呈正相关(r=0.593,0.516,0.549,均P=0.001)。结论 MCP-3,NRG-1β和CF6在慢性心力衰竭患者中表达升高,且MCP-3,NRG-1β和CF6表达升高与患者疾病严重程度相关,可参与慢性心力衰竭的发展。临床可参考上述指标对此病进行早期评估。

Objective To analyze the serum monocyte chemoattractant protein-3(MCP-3),neuregulin 1β(NRG-1β)and coupling factor 6(CF6)levels in patients with chronic heart failure(CHF)and their correlation with the severity of the disease.Methods From August 2019 to August 2020,120 patients with chronic heart failure admitted to the eastern district of the First Hospital of Zhangjiakou City as the CHF group,and 70 healthy people were selected as the healthy group,using enzyme-linked immunoassay(ELISA)method to detect the levels of MCP-3,NRG-1β and CF6,and analyzed the expression of MCP-3,NRG-1β and CF6 in the healthy group and the CHF group and the relationship with the severity of the disease.Results The expressions of MCP-3(0.52±0.05 ng/L),NRG-1β(6.01±0.62 ng/ml),and CF6(304.64±30.58 pg/ml)in the CHF group were higher than those in the healthy group(0.10±0.01 ng/L,3.25±0.32 ng/ml,201.62±21.36 pg/ml),the differences were statistically significant(t=69.400,34.630,24.860,all P<0.05).MCP-3,NRG-1β and CF6 increased with the severity of CHF the differences were statistically significant(F=15.117~41.816,all P<0.05).The ROC curve showed that compared with the single diagnosis of MCP-3(AUC=0.842,95% CI=0.701~0.984),NRG-1β(AUC=0.851,95% CI=0.718~0.984)and CF6(AUC=0.873,95% CI=0.746~1.000),the combination of the three had a higher diagnostic value for CHF(AUC=0.899,95%CI=0.766~1.030),the differences were statistically significant(all P<0.05).Correlation analysis showed that MCP-3 was positively correlated with NRG-1β and CF6 and NRG-1β was positively correlated with CF6(r=0.593,0.516,0.549,all P=0.001).Conclusion The expression of MCP-3,NRG-1β and CF6 were increased in patients with CHF,and the increased expression of MCP-3,NRG-1β and CF6 was related to the severity of the patient’s disease,and may participate in the development of CHF.Clinical can refer to the above indicators for early evaluation of the disease.