^(1)O_(2)-activatable Eu^(3+)-afterglow nanoprobe for highly sensitive detection of porphyria in whole blood

High-sensitivity detection of porphyrin in blood is very important for the early diagnosis and treatment of porphyria.Based on the advantages of longer luminescence lifetime and lower background interference,organic afterglow molecular porphyrin detection probes were developed,but these probes show poor water solubility and insufficient luminescence intensity.Herein,we present an afterglow nanoprobe(Eu-NP)for porphyria detection in whole blood.The luminescent substance(europium complex)and the organic vinyl co mpound reacted with singlet oxygen(^(1)O_(2))were selected for encapsulation in polystyrene spheres.Eu-NP can respond to^(1)O_(2)produced by porphyrins,and the detection signals can be obtained by the emission of the afterglow Eu^(3+)-emissive nanosensor.Eu-NP can achieve high-sensitivity detection of porphyrin,and the lowest detection limit reaches 0.29μmol/L.The porphyrin detection in whole blood samples is consistent with clinical diagnosis results.Compared with the organic afterglow molecule,the Eu-NPs constructed in this work show a higher signal-to-noise ratio and sensitivity of porphyria detection.