管花肉苁蓉提取物对D-半乳糖致衰老模型小鼠的影响

Effects of Cistanche deserticola Extract on D-Galactose-Induced Aging Model Mice

探究管花肉苁蓉提取物对D-半乳糖致衰老小鼠抗氧化作用,将实验动物分为空白组、模型组、V_(C)阳性组(1.70 mg/10 g)、管花肉苁蓉提取物低(1 mg/10 g)、中(2 mg/10 g)、高(4 mg/10 g)剂量组,持续灌胃给药30 d,测定肝、脑组织脏器指数以及各组动物肝、脑组织和血清中谷胱甘肽过氧化物歧化酶(glutathione peroxide dismutase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)活性及丙二醛(malondialdehyde,MDA)含量;HE染色肝、脑组织并观察结构的变化。结果表明,与模型组相比,给药组动物体质量均高于模型组。管花肉苁蓉提取物低、中、高剂量组及V_(C)阳性组肝、脑脏器指数升高(P<0.05),中剂量组小鼠肝、脑脏器指数升高最为明显,分别为模型组的1.62和2.31倍。管花肉苁蓉提取物低、中、高剂量组肝、脑组织及血清中GSH-Px和SOD的活性均显著升高(P<0.05),低剂量肝组织、中剂量脑组织、高剂量血清中GSH-Px活性升高最为明显分别为模型组的1.37、1.18、1.46倍,中剂量肝组织、高剂量脑组织、中剂量血清中SOD活性升高最为明显分别为模型组的1.11、1.14、1.74倍。管花肉苁蓉提取物中、高剂量组肝、脑组织及血清中MDA含量均极显著降低(P<0.01),中剂量肝组织、高剂量脑组织、中剂量血清中MDA含量降低最为明显分别比模型组减少23.86%、41.68%和38.30%。HE染色发现管花肉苁蓉提取物对衰老导致小鼠肝、脑组织细胞及结构损伤具有保护作用。因此,推测管花肉苁蓉提取物对D-半乳糖致衰老模型小鼠有较好的保护作用。

To investigate the antioxidant effect of Cistanche deserticola extract on D-galactose in aging mice,the experimental animals were divided into blank group,model group,V_(C) positive group(1.70 mg/10 g),low(1 mg/10 g),medium(2 mg/10 g)and high(4 mg/10 g)dose groups of Cistanche deserticola extract which were administered by gavage for 30 d.The organ indices of liver and brain tissues as well as the activities of glutathione peroxide dismutase(GSH-Px)and superoxide dismutase(SOD)and malondialdehyde(MDA)content in liver and brain tissues and animal serum were determined among each group.The structural changes of liver and brain tissues were observed by hematoxylin-eosinstaining(HE).The results showed that comparing with the model group,the mass of animals in the medication administration group was higher than that in the model group.The indexes of liver and brain organs increased in the low,medium and high dose groups of Cistanche tubulosa extract and V_(C) positive group(P<0.05).The liver and brain organ indexes of mice in the medium dose group increased most significantly,which were 1.62 and 2.31 times that of the model group.The activities of GSH-Px and SOD of liver,brain tissue and serum of Cistanche tubulosa extract in low,medium and high dose groups increased significantly(P<0.05).The activity of GSH-Px in low-dose liver tissue,medium-dose brain tissue,and high-dose serum increased most significantly,which were 1.37,1.18,and 1.46 times of the model group,respectively.The activities of SOD of medium dose liver tissue,high dose brain tissue,and medium dose serum increased and the most obvious were 1.11,1.14,and 1.74 times of the model group,respectively.Liver and brain tissue in the medium and high dose groups of Cistanche tubulosa extract and MDA in serum decreased significantly(P<0.01).Compared with the model group,the MDA of the medium dose liver tissue,high dose brain tissue,medium dose serum decreased most significantly,which decreased by 23.86%,41.68% and 38.30% respectively.It was found that Cistanche deserticola extract had a protective effect on cellular and structural damage in liver and brain tissues of mice caused by aging.Therefore,it was hypothesized that Cistanche deserticola extract had a good protective effect on D-galactose-induced senescence model mice.