摘要
种衣剂有效成分的残留分布和降解行为可影响其使用方法、作用效果和适用范围,也是了解药剂持效期和残留风险的数据基础。本研究建立了大豆植株和根围土壤中精甲霜灵和咯菌腈的HPLC分析方法,并采用该方法研究了6.25%精甲·咯菌腈悬浮种衣剂中的2种有效成分精甲霜灵和咯菌腈在大豆种子、根、叶和根围土壤中的残留降解动态和分布。结果显示,在0.35~50.0 mg/L(精甲霜灵)和0.15~50.0 mg/L(咯菌腈)范围内,2种有效成分的质量浓度与其各自峰面积均呈良好的线性关系(R^(2)>0.99)。在0.10~5.00 mg/kg添加水平下,精甲霜灵在大豆和土壤样品中的平均回收率为84%~97%;咯菌腈在大豆和土壤样品中的平均回收率为80%~95%,相对标准偏差(RSD)均小于7%。两者的降解行为符合一级动力学或一级累积-消解动力学模型,半衰期为1.097~103.2 d。在种植6.25%精甲·咯菌腈悬浮种衣剂包衣的大豆种子后,2种有效成分初期主要累积于根部和种子,随后可被传导或扩散至叶部,咯菌腈在根部和叶片的占比低于精甲霜灵;也可被扩散至根围土壤中,咯菌腈的扩散性能优于精甲霜灵。综上所述,2种有效成分均可被大豆植株吸收、传导或扩散来发挥保护作用。本研究可为该药剂的科学合理使用提供依据。
The residual distribution and degradation behaviors of the active ingredients of a seed-coating agent are the key factors affecting its application method,efficacy and applicable scope,as well as the data basis for understanding of its lasting period and residual risk.In this study,a HPLC analytical method of metalaxyl-M and fludioxonil in soybean plants and rhizosphere soil was established.Meanwhile,the residual degradation dynamics and distribution of the two active ingredients of 6.25% metalaxyl-M·fludioxonil FSC in soybean seeds,roots,leaves and rhizosphere soil were studied.The results showed that there were good linear correlations(R^(2)>0.99) between the mass concentrations of two active ingredients and their peak areas in the range of 0.35-50.0 mg/L(metalaxyl-M) and 0.15-50.0 mg/L(fludioxonil),respectively.At the spiking levels of 0.10-5.00 mg/kg,the average recoveries of metalaxyl-M in soybean and soil samples were 84%-97%,and the average recoveries of fludioxonil in soybean and soil samples were 80%-95%.The relative standard deviations(RSD) were all less than 7%.Furthermore,the degradation behaviors of the two active ingredients were in accordance with the first-order kinetic equation or the first-order accumulation-dissipation kinetics model,and half-lives were 1.097 ~103.2 d.After the soybean seeds were treated with 6.25% metalaxyl-M·fludioxonil FSC,the two active ingredients mainly accumulated in roots and seeds at the early stage,being subsequently transmitted or spread to leaves.The proportion of fludioxonil in roots and leaves was lower than that of metalaxyl-M.The two active ingredients were also capable of being diffused into soil,and the diffusion property of fludioxonil was better than that of metalaxyl-M.In summary,the two active ingredients could be absorbed,transmitted or diffused by soybean plants to play a protective role.The findings provided reference for the scientific and rational use of seed-coating agent.
作者
邢小霞
于鑫
张清明
李平亮
XING Xiaoxia;YU Xin;ZHANG Qingming;LI Pingliang(College of Plant Health and Medicine,Qingdao Agricultural University,Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management,Shandong Qingdao266109,China)
出处
《现代农药》
CAS
2022年第5期27-33,共7页
MODERN AGROCHEMICALS
基金
国家科技部重点研发计划(2017YFD0201603)
山东省自然科学基金青年项目(ZR2020QC137)
青岛农业大学博士启动基金(663/1114318)。
关键词
大豆
种衣剂
精甲霜灵
咯菌腈
降解动态
根围土壤
soybean
seed-coatin agent
metalaxyl-m
fludioxonil
degradation dynamic
rhizosphere soil