基于网络药理学研究通气散干预分泌性中耳炎作用机制

Study on the mechanism of Tongqi Powder in the treatment of otitis media with effusion based on network pharmacology

目的通过网络药理学研究通气散治疗分泌性中耳炎的作用机制。方法运用TCMSP平台筛选通气散的有效成分及靶蛋白,通过UniProt数据库映射得到通气散的作用靶点基因。基于OMIM、DisGeNET、GeneCards数据库获取分泌性中耳炎相关靶点,找到药物和疾病的共同靶点即为通气散作用于分泌性中耳炎的预测靶点,导入Cytoscape软件,得到药物-成分-靶点-疾病网络。将重复靶点导入STRING平台,构建蛋白质-蛋白质相互作用网络。利用Metascape平台对通气散作用靶点进行GO富集分析和KEGG通路富集分析。结果获得通气散有效成分37个,靶点211个,关键有效成分有槲皮素、山柰酚、木犀草素、β-谷甾醇等。疾病靶点1431个,药物疾病共同作用靶点76个,关键靶点有TNF、转录因子AP-1、α丝氨酸/苏氨酸蛋白激酶等。富集分析得到273个信号通路。结论通气散可通过晚期糖基化终产物AGE-RAGE、流体剪应力与动脉粥样硬化、癌症途径、IL-17、TNF等信号通路调节分泌性中耳炎的疾病进程。

Objective To study the mechanism of Tongqi Powder in the treatment of Otitis Media with Effusion based on network pharmacology.Methods TCMSP platform was used to screen out the effective components and targeted proteins of Tongqi Powder,and UniProt database was used to map the targeted genes of it.The related targets of Otitis Media with Effusion were obtained based on OMIM,DisGeNET,GeneCards and other databases,finding that the common target of Tongqi Powder and Otitis Media with Effusion was the predicted target,Then,a medicine-component-target-disease network was obtained by using Cytoscape software.Repeated targets were imported into the STRING platform to construct a PPI network of protein interactions.Go enrichment analysis and KEGG pathway enrichment analysis were performed on the target of Tongqi Powder by Metascape platform.Results There were 37 effective components and 211 targets of Tongqi Powder,and the key active components included quercetin,kaempferol,luteolin,β-sitosterol,etc.There were 1431 disease targets,76 co-acting targets of Tongqi Powder and Otitis Media with Effusion,and the key targets included TNF,JUN,AKT1,etc.A total of 273 signal pathways were obtained by enrichment analysis.Conclusion Tongqi Powder could suppress the development of Otitis Media with Effusion through AGE-RAGE,fluid shear stress,atherosclerosis,cancer pathway,IL-17,tumor necrosis factor and other signaling pathways.