两亲性胶束包覆Eu^(3+)掺杂LDHs纳米载体材料的制备与性能研究

Preparation and properties of Eu^(3+)-doped LDHs nano carrier materials coated by amphiphilic micelles

采用两亲性胶束CSA-ss-DOCA(CsD)对Eu^(3+)掺杂层状双氢氧化物Eu-LDH(LE)进行包覆,制备得到纳米载体材料LE@CsD。利用荧光光谱、FT-IR、XRD、TG、粒度以及溶血实验和蛋白吸附等方法对样品结构、尺寸、热力学性质与生物相容性进行表征。结果表明,LE@CsD在613 nm处(λ_(ex)=306 nm)具有Eu^(3+)的特征荧光,CsD对LE的包覆并未影响样品获得荧光特性;模板药物分子五氟尿嘧啶(5Fu)被成功负载到LE@CsD上,且其层状结构未受破坏,验证了样品的载体性质;负电性胶束CsD通过静电作用与LE结合,"屏蔽"了LE层板的正电荷,使LE@CsD的蛋白吸附率仅为0.05%,溶血率低至1.16%,平均粒径控制为(230.3±2.7)nm,具备良好的生物相容性。

LE@CsD,a kind of nano carrier material,is prepared by using amphiphilic micelles CSA-ss-DOCA to coat Eu^(3+)-doped layered double hydroxides(Eu-LDH,or LE).The structure,size,thermodynamic properties and biocompatibility of the samples are investigated by means of fluorescence spectra,FT-IR,XRD,TG,particle size analyzer,hemolysis test and protein adsorption.The results indicate that LE@CsD has a characteristic fluorescence of Eu^(3+)at 613 nm(λ_(ex)=306 nm),and the fluorescence characteristics of the sample is not affected by the coating of LE by CsD;Pentafluorouracil(5Fu),the template drug molecule,is successfully loaded onto LE@CsD,and its layered structure has not been damaged,verifying the carrier properties of the sample;CsD negative micelles combines with LE through electrostatic action to"shield"the positive charge of LE laminate,which makes the protein adsorption rate as low as 0.05%,the hemolysis rate as low as 1.16%,and the average particle size be controlled at 230.3±2.7 nm.LE@CsD also has a good biocompatibility.